CD147和CK19在肝细胞癌中的表达及临床意义

目的 探讨细胞外基质蛋白诱导因子（CD147）和细胞角蛋白19（CK19）在肝细胞癌（HCC）中的表达及临床意义。方法 采用组织芯片技术和免疫组织化学法检测CD147和CK19在272例HCC组织和81例癌旁组织中的表达情况。结果 CD147在HCC中的阳性表达率为73.53％（200／272），在癌旁组织中的阳性表达率为13.58％（11／81），差异有统计学意义（P＜0.05）。CK19在HCC组织中的阳性表达率为14.34％（39／272），CK19在癌旁组织中无表达。在HCC中，CD147的表达与组织学分级、临床分期、术后无瘤生存时间、肿瘤直径、脉管或门静脉癌栓相关，与性别、年龄、肝硬化、AFP水平、HBV感染、淋巴结转移、病灶数目、肝被膜浸润及卫星灶无关（P＞0.05）；CK19在HCC中的表达与术后无瘤生存时间、组织学分级、肿瘤直径、肝硬化、卫星灶、淋巴结转移及临床分期有关，与性别、年龄、病灶数目、肝被膜浸润、AFP水平、HBV感染及脉管或门静脉癌栓无关（P＞0.05）。在HCC中，CD147 阳性表达者与阴性表达者的中位复发时间分别为13个月和48个月（P＜0.05），中位生存时间分别为24个月和60个月（P＜0.05）；CK19阳性表达者与阴性表达者的中位复发时间分别为7个月和31个月（P＜0.05），中位生存时间分别为13个月和42个月（P＜0.05）。 CD147的表达和CK19的表达无明显相关性（r=0.061，P=0.317）。结论 HCC中CD147和CK19的表达与预后密切相关，且两者均可作为HCC预后不良的判断指标。

Expression and clinical significance of CD147 and CK19 in hepatocellular carcinoma

Objective To investigate the expression of CD147 and CK19 in hepatocellular carcinoma（HCC） and their clinical significance.Methods The expression of CD147 and CK19 was determined by tissue microarray and immunohistochemistry in 272 cases of HCC and 81 cases of adjacent tumorous tissue.Results The positive expression of CD147 in HCC and adjacent tumorous tissue was 73.53%（200/272）and 13.58%（11/81） with significant difference（P0.05）.The positive expression of CK19 of HCC and adjacent tumorous tissue was 14.34%（39/272） and 0.The positive expression of CD147 was closely correlated to the histological grade,clinical stage,tumor-free survival,diameter of tumor and embolus of cancer in aqueduct or portal vein;but not to patients＇ sex,age,liver cirrhosis,AFP level,infection of HBV,lymph node metastasis,number of tumor,invasion of liver involucrum and micro-satellites.The expression of CK19 in HCC was closely correlated to the tumor-free survival,histological grade,diameter of tumor,liver cirrhosis,micro-satellites,lymph node metastasis and clinical stage;but not to patients＇ sex,age,number of tumor,invasion of liver involucrum,AFP level,infection of HBV and embolus of cancer in aqueduct or portal vein.Among the patients of positive expression of CD147,the median replacing time and survival were 13 and 24 months,lower than 48 and 60 months in the patients of negative expression（P0.05）.Among the patients of positive expression of CK19,the median replacing time and survival were 7 and 13 months,lower than 31 and 42 months in the patients of negative expression（P0.05）.The expression of CD147 had no correlation with CK19（r=0.061,P=0.317）.Conclusion The expression of CD147 and CK19 closely correlate with the clinical prognosis of HCC,which may indicate poor prognosis of HCC.