Differentiation of stem cells from human deciduous and permanent teeth into spiral ganglion neuron-like cells |
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| Affiliation: | 1. Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand;2. Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand;3. Department of Oral Biology, Faculty of Dentistry, Mahidol University, 6 Yothi Road, Rajthawee, Bangkok, 10400, Thailand;1. Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Carl Neuberg-Str. 1, 30625 Hannover, Germany;2. Department of Trauma Surgery, Hannover Medical School, Carl Neuberg-Str. 1, 30625 Hannover, Germany;3. Department of Orthopaedic Surgery, Hannover Medical School, Annastift, Anna von Borries-Str. 1-7, 30625 Hannover, Germany;4. Helmholtz Centre for Infection Research, Department of Gene Regulation and Differentiation, Inhoffenstr. 7, 38124 Braunschweig, Germany;5. Cluster of Excellence “Hearing4all” of the German Research Foundation, Germany;1. Dept. Chemical & Biomedical Engineering, Global Center for Hearing & Speech Research, University of South Florida, Tampa, FL, USA;2. Dept. Communication Sciences & Disorders, Global Center for Hearing & Speech Research, University of South Florida, Tampa, FL, USA;3. Dept. Medical Engineering, Global Center for Hearing & Speech Research, University of South Florida, Tampa, FL, USA;4. Small Animal Imaging Lab, Moffitt Cancer Center, Tampa, FL, USA;5. Microsystems Engineering, Rochester Institute of Technology, Rochester, NY, USA;1. Department of Otorhinolaryngology – Head and Neck Surgery, Kresge Hearing Research Institute, The University of Michigan Medical School, Ann Arbor, Michigan, USA;2. Department of Otorhinolaryngology – Head and Neck Surgery, Dankook University Hospital, Cheonan, South Korea;1. Department of Fixed Prosthesis & Implant Prosthodontics, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki GR-54124, Greece;2. Department of Conservative Dentistry, Periodontology & Preventive Dentistry, School of Dentistry, Hannover Medical School, Hannover D-30625, Germany;1. University of Zurich, University Hospital Zurich, Department of Otorhinolaryngology, Head and Neck Surgery, Frauenklinikstrasse 24, 8091, Zurich, Switzerland;2. Institute of Fluid Dynamics, Swiss Federal Institute of Technology (ETHZ), Sonneggstrasse 3, 8092, Zurich, Switzerland;3. Streamwise GmbH, Emil-Staub-Strasse 5, 8708, Männedorf, Switzerland;4. University of Zurich, University Hospital Zurich, Institute of Diagnostic and Interventional Radiology, Rämistrasse 100, 8091, Zurich, Switzerland;5. University of Zurich, Institute for Evolutionary Medicine, Winterthurerstrasse 190, 8057, Zurich, Switzerland |
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| Abstract: | ObjectiveStem cells from pulp tissue are a promising cell-based therapy for neurodegenerative patients based on their origin in the neural crest. The aim of this study was to differentiate and evaluate the ability of human dental pulp stem cells from permanent teeth (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) to differentiate into spiral ganglion neurons.DesignAfter isolation and characterization of mesenchymal stem cell properties, DPSC and SHED were treated with the neurotrophins brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and glial cell-derived neurotrophic factor (GDNF). The differentiation was identified by immunostaining and qRT-PCR analysis of neuronal markers and measuring intracellular calcium activity.ResultsAfter 2 weeks of induction, morphological changes were observed in both DPSC and SHED. The differentiated cells expressed neuron-specific class III beta-tubulin, GATA binding protein 3 (GATA3) and tropomyosin receptor kinase B, protein markers of spiral ganglion neurons. These cells also showed upregulation of the genes encoding these proteins, namely GATA3 and neurotrophic receptor tyrosine kinase 2. Intracellular calcium dynamics that reflect neurotransmitter release were observed in differentiated DPSC and SHED.ConclusionThese results demonstrate that dental pulp stem cells from permanent and deciduous teeth can differentiate into spiral ganglion neuron-like cells. |
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| Keywords: | Dental pulp stem cells Stem cells from human exfoliated deciduous teeth (SHED) Spiral ganglion neuron Neurotrophins |
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