Effect of sodium fluoride on oral biofilm microbiota and enamel demineralization |
| |
| Affiliation: | 1. Division of Oral Microbiology and Immunology, Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zürich, Zürich, Switzerland;2. Division of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden;1. São Paulo State University (Unesp), School of Dentistry, Araçatuba, Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193, 16015-050, Araçatuba, SP, Brazil;2. LIEC-Department of Chemistry, Federal University of São Carlos (UFSCar), 13565-905, São Carlos, SP, Brazil;1. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil;2. Department of Orofacial Sciences, School of Dentistry, University of California, San Francisco, San Francisco, USA;3. Department of Morphophysiological Sciences, State University of Maringá, Maringá, Brazil;1. Department of Operative Dentistry, Faculty of Oral and Dental Medicine, Misr International University, Egypt;2. Department of Pediatric Dentistry and Dental Public Health, Faculty of Oral and Dental Medicine, Misr International University, Egypt;1. Faculty of Dentistry, Beirut Arab University, Beirut, Lebanon;2. Department of Pediatric Dentistry, Faculty of Dentistry, Beirut Arab University, Beirut, Lebanon;3. Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt;4. Department of Orthodontics, Faculty of Dentistry, Beirut Arab University, Beirut, Lebanon;5. Alexandria University, Alexandria, Egypt;1. Department of Paediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;2. Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;3. School of Pharmacy, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;4. Laboratório Experimental de Drogas Antivirais e Citotóxicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;5. Department of Clinic and Social Dentistry, School of Dentistry, Universidade Federal da Paraíba, Paraíba, Brazil;1. King Saud University, College of Applied Medical Sciences, Dental Health Department, P.O. Box 145111, Riyadh, 4545, Saudi Arabia;2. Indiana University, School of Dentistry, Department of Biomedical Sciences and Comprehensive Care, 1121 W. Michigan Street, Indianapolis, IN, 46202, USA;3. Indiana University, School of Medicine, Department of Family Medicine, Bowen Center for Health Workforce Research & Policy, 1110 W. Michigan Street, Indianapolis, IN, 46202, USA;4. Indiana University, School of Medicine, Department of Biostatistics, 410 W. 10th Street, HITS 3000, Indianapolis, IN, 46202, USA;5. Indiana University, School of Dentistry, Department of Cariology, Operative Dentistry and Dental Public Health, 415 Lansing Street, Indianapolis, IN, 46202, USA |
| |
| Abstract: | ObjectiveFluoride is widely used as an anti-caries agent, e.g. in toothpastes and mouth rinses. However, the nature of the anti-caries action is not entirely clear. Mechanisms suspected to explain the cariostatic effect include inhibitory effects on acid formation by bacteria, inhibition of extracellular polysaccharide (EPS) production, inhibition of enamel demineralization and enhancement of remineralizaton or combination thereof. The aim of this study was to examine with the supragingival Zurich in vitro biofilm model the effect of fluoride in NaF formulation, on the microbiota and on demineralization.MethodsBiofilms consisting of Actinomyces oris, Candida albicans, Fusobacterium nucleatum, Streptococcus oralis, Veillonella dispar and Streptococcus sobrinus, were grown anaerobically on sintered hydroxyapatite or bovine enamel disks, exposed to 200, 400, and 1400 ppm of NaF, or 0.1% chlorhexidine (positive control). The biofilms were harvested after 64 h and CFUs were assessed for total bacteria. Demineralization of enamel disks was measured by quantitative light-induced fluorescence.ResultsNaF did not affect the bacterial numbers. No enamel mineral loss was observed at 1400 and 400 ppm of fluoride, whereas the pH of the surrounding medium was increased to 5.5 and 5.0, respectively, compared to the untreated control (pH 4.5 and mineral loss ΔF of −32%). At 1400 ppm NaF the biofilm’s EPS volume was also significantly reduced.ConclusionsAdministration of NaF completely prevented demineralization without affecting biofilm composition and growth. This protective effect may be attributed to the observed decrease in acid production or EPS volume, or to a shift in the de/remineralization balance. |
| |
| Keywords: | Biofilms Fluoride NaF Enamel Demineralization Quantitative light-induced fluorescence (QLF) |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
