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| HPLC法测定血康颗粒中芍药苷的含量 | |
| 引用本文： | 程东方,谢纪珍,贾元印. HPLC法测定血康颗粒中芍药苷的含量[J]. 齐鲁药事, 2013, 32(3): 150-152 |
| 作者姓名： | 程东方 谢纪珍 贾元印 |
| 作者单位： | 程东方 (山东中医药大学,山东,济南,250355)； 谢纪珍 (山东省药品审评认证中心,山东,济南,250014)； 贾元印 (山东省中医药研究院,山东,济南,250014)； |
| 摘 要： | 目的建立高效液相色谱法测定血康颗粒中芍药苷的含测方法。方法采用HPLC-ELSD法,用十八烷基硅烷键合硅胶为填充剂;以乙腈-0.1%磷酸溶液(14∶86)为流动相;检测波长231 nm,对样品中的芍药苷进行测定。结果通过方法学的系统考察和三批样品的含量测定,建立了制剂中芍药苷的高效液相色谱含测方法。其回归方程为Y=1 261 660.7X-578.7,r=0.999 8。结论建立的含量测定方法,具有灵敏度高、专属性强等特点,是控制本品内在质量的较理想方法。 |
| 关 键 词： | 血康颗粒 高效液相色谱 芍药苷 含量测定 |
Determination of paeoniflorin in Xuekang Granules by HPLC |
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| CHENG Dong-fang,XIE Ji-zhen,JIA Yuan-yin. Determination of paeoniflorin in Xuekang Granules by HPLC[J]. qilu pharmaceutical affairs, 2013, 32(3): 150-152 | |
| Authors: | CHENG Dong-fang XIE Ji-zhen JIA Yuan-yin |
| Affiliation: | 1.Shandong University of Traditional Chinese Medicine,Jinan 250355,China;2.Certification Center of Shandong Province for Drug Evaluation,Jinan 250014,China;3.Shandong Academy of Chinese Medicine,Jinan 250014,China) |
| Abstract: | Objective To establish a method for the determination of paeoniflorin in Xuekang Granules by HPLC.Methods The content determination used HPLC-ELSD,chromatographic conditions were carried out as following:column was packed with the octadecyl silane chemically bonded silica,flowing phase was composed with acetonitrile and 0.1% phosphoric acid(14∶ 86)and the detection wavelength was 231 nm.Results The HPLC method for determination of paeoniflorin in this preparation was established through methodology of systematic investigation and determination of three batch sample.The regression equation was Y=1 261 660.7X-578.7,r=0.999 8.Conclusion The method was sensitive,highly specific,and a the ideal way to control the quality of the products. |
| Keywords: | Xuekang Granules HPLC Paeoniflorin Determination |
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