Identification of receptors for fibrinogen and von Willebrand factor mediating aggregation in guinea pig platelets |
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| Authors: | J M Kupinski J L Miller |
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| Affiliation: | 1. Faculty of Engineering and Natural Sciences, Bahcesehir University, Istanbul, 34353, Turkey;2. Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan;3. Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, 161200, Kazakhstan;4. Institute of Basic Biological Problems, FRC PSCBR RAS, Pushchino, Moscow Region, 142292, Russia;5. Department of Biology, Faculty of Technology, Taraz Regional University Named After M.Kh.Dulaty, 7 Suleimenov, Str, 080000, Taraz, Kazakhstan;6. Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia;7. K.A. Timiryazev Institute of Plant Physiology, RAS, Botanicheskaya Street 35, Moscow, 127276, Russia;1. Department of Civil Engineering, College of Engineering, University of Duhok, Duhok, KR, Iraq;2. Department of Civil Engineering, College of Engineering, Nawroz University, 42001 Duhok, Iraq;3. Department of Civil Engineering, Université de Sherbrooke, 2500 Blvd. de L''Université, Sherbrooke, QC J1K 2R1, Canada;4. Civil Engineering Department, Faculty of Engineering, Islamic University of Gaza, P.O. Box 108, Gaza Strip, Palestine;5. Civil Engineering Department, College of Engineering, University of Sulaimani, Sulaimaniyah, KR, Iraq;6. Civil Engineering Department, University of Halabja, Halabja, Kurdistan Region, Iraq;1. Department of Mechanical Systems Engineering, Tokyo University of Agriculture and Technology, Koganei Campus 6–507, 2–24-16 Nakacho, Koganei, Tokyo, Japan;2. Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Fuchu Campus, 3–5-8 Saiwaicho, Fuchu, Tokyo, Japan;1. Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, Poznan, 60-695, Poland;2. Department of Physics and Biophysics, Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 38/42, 60-637, Poznań, Poland |
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| Abstract: | ![]()
Monoclonal antibodies were prepared to guinea pig platelets and selected for their ability to inhibit ADP-induced platelet aggregation and ristocetin induced, Ca++-independent platelet agglutination. One antibody, PG-2, produced strong inhibition of aggregation induced by ADP, thrombin, collagen and arachidonic acid, while not inhibiting ristocetin-induced agglutination. A second antibody, PG-1, blocked ristocetin-induced agglutination, but did not inhibit aggregation induced by the previous agents. PG-2 blocked ADP-induced 125I-fibrinogen binding to washed guinea pig platelets by approximately 50%, but did not inhibit ristocetin-induced binding of 125I-vWF. Conversely, PG-1 selectively inhibited ristocetin-induced 125I-vWF binding, with the degree of inhibition inversely related to the ristocetin concentration. These studies suggest that in guinea pig platelets, fibrinogen and von Willebrand factor binding to different membrane sites are responsible for the aggregation response of stimulated platelets and the ristocetin-induced agglutination response respectively. These antibodies offer significant promise for the further development of a guinea pig animal model for studying platelet and megakaryocyte function. |
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