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| 反相高效液相色谱法测定醋酸奥曲肽中奥曲肽含量 | |
| 引用本文： | 沈显威,刘百川,刘积平,徐红岩,曲莉莉. 反相高效液相色谱法测定醋酸奥曲肽中奥曲肽含量[J]. 武警医学院学报, 2012, 21(4): 251-252 |
| 作者姓名： | 沈显威 刘百川 刘积平 徐红岩 曲莉莉 |
| 作者单位： | 1. 武警吉林总队医院医务处,吉林长春,130052 2. 长春普莱医药生物技术有限公司,吉林长春,130052 |
| 摘 要： | [目的]建立高效液相色谱法测定醋酸奥曲肽中奥曲肽含量的方法.[方法]色谱柱:ZORBAX 300SB-C8柱(2.1mm×150mm,5μm),流动相:A:0.1％TFA水B:0.1％TFA乙腈,流速:0.25 ml/min,柱温:40℃,检测波长:210nm,进样量:20μl.[结果]奥曲肽浓度在0.08 ～ 0.2 mg/ml范围内,线性关系良好,r=0.9999;检测限为0.02 mg/ml;加样回收率平均为101.28％,RSD为0.58％.[结论]分析方法可行,准确度高,此法可用于醋酸奥曲肽中奥曲肽含量的测定. |
| 关 键 词： | 醋酸奥曲肽 高效液相色谱法 奥曲肽含量 |
Determine of the content of octreotide in octreotide acetate by HPLC method |
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| SHEN Xian-wei , LIU Bai-chuan , LIU Ji-ping , XU Hong-yan , QU Li-li. Determine of the content of octreotide in octreotide acetate by HPLC method[J]. Acta Academiae Medicinae CPAPF, 2012, 21(4): 251-252 | |
| Authors: | SHEN Xian-wei LIU Bai-chuan LIU Ji-ping XU Hong-yan QU Li-li |
| Affiliation: | (Department of Medical Service,Jilin Provincial Corps Hospital of Chinese People’s Armed Police Force,Changchun 130052,China) |
| Abstract: | 【Objective】To establish a HPLC method for the determination of the content of octreotide in octreotide acetate.【Methods】ZORBAX 300SB-C8 column(2.1mm×150 mm,5μm) and 0.1%TFA water 0.1%TFA acetonitrile as the mobile phase at a flow rate of 0.25ml/min were used with a column temperature of 40℃,a detection wavelength of 210 nm and an injection volume of 20 μl.【Results】The standard curve of octreotide was linear in the range of 0.08～0.20 mg/ml,r=0.9999.The detection limit was 0.02 mg /ml.The average recovery rate was 101.28%,and RSD was 0.58%.【Conclusion】The method is practicable and accurate and can be used for the determination of the content of octreotide in octreotide acetate. |
| Keywords: | Octreotide acetate HPLC Content of octreotide |
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