腺苷在小鼠脑组织中的分布

目的:监测小鼠脑组织中的腺苷含量,探讨腺苷在各脑区中的分布。方法:雄性昆明种小鼠8只,在乙醚麻醉下断头处死,迅速取出脑组织并分离出皮层、海马、纹状体、丘脑、下丘脑、中脑等脑区。这些脑组织被制成匀浆,采用高效液相色谱-紫外(HPLC-UV)检测法检测其腺苷含量。结果:腺苷在小鼠纹状体、皮层及海马中含量较高,下丘脑和丘脑次之,中脑最低。结论:腺苷含量在小鼠各脑区中呈不均匀分布,其中纹状体内含量最高。

Distribution of adenosine in the brain of mice

Objective: To detect adenosine contents in brain homogenates for investigating the distribution of adenosine in the brain of mice.Methods: Eight male mice of Kunming strain were killed by decapitation under anesthesia by diethyl ether,and the brain tissues were quickly removed out and divided on ice into seven regions: cortex,striatum,hippocampus,hypothalamus,thalamus and midbrain.These brain tissues were homogenized and determined for the adenosine content by HPLC with ultraviolet detector.Results: The adenosine level was the highest in striatum,cortex and hippocampus,moderately downward for hypothalamus and thalamus,and the lowest in midbrain of the mouse brain.Conclusion: Adenosine content is unevenly distributed in different cerebral regions of mice in which the striatum holds maximally.