鸡胆粉HPLC指纹特征研究

目的:探求土鸡胆粉与肉鸡胆粉的HPLC指纹特征,为建立鸡胆粉的色谱指纹图谱提供依据。方法:采用梯度洗脱技术进行HPLC分离分析。色谱柱为Lichrospher C18(4.6 mm×250 mm,5μm),以乙腈-0.001 mol/L KH2PO4溶液为流动相,梯度洗脱(0～20 min,乙腈30%→30%;20～40 min,乙腈30%→40%;40～70 min,乙腈40%→50%),流速:1.0 ml/min;柱温30℃;DAD检测器,检测波长为210nm。用聚类分析方法处理色谱指纹信息,比较色谱以鹅去氧胆酸为参照色谱峰的18个特征峰的信息。结果:通过分析10批样品,确定了18个共有峰,建立了鸡胆粉的HPLC指纹特征图谱。结论:土鸡胆粉与肉鸡胆粉的色谱指纹特征比较相近,但特征成分间含量有一定的差异。

Study on HPLC fingerprint characteristic of the chicken bile powder

Objective:To investigate the HPLC fingerprint characteristics of the bile powder from native chicken and broilers for basis to establish the chromatographic fingerprint of chicken bile powder.Methods:The HPLC was run on Lichrospher C18(4.6 mm×250 mm,5 μm) column with acetonitrile-water(0.001 mol/L potassium dihydrogen phosphate) as mobile phase in gradient elution for 0 to 20 min(acetonitrile 30%→30%),20 to 40 min(acetonitrile 30%→40%) and 40 to 70 min(acetonitrile 40%→50%),respectively,at a flow rate of 1.0 ml/min and at temperature of column by 30 ℃.DAD detector was set at 210 nm to detect the wavelength and reference wavelength.The information of 18 fingerprint peaks compared with chenodeoxycholic acid were treated with cluster analysis method.Results:By analysis of 10 batches of samples,18 peaks were identified and the HPLC fingerprint of the chicken bile powder was established.Conclusion:HPLC fingerprint characteristic of the bile powder from native chicken resembles that of broilers,yet,the content among the characteristic ingredients displays somewhat differently.