多指标正交试验优选莲松异搏停片中延胡索、苦参醇提工艺

目的： 优选莲松异搏停片中延胡索、苦参的提取工艺。 方法： 以延胡索乙素、苦参碱和氧化苦参碱提取量为指标，通过正交试验和单因素试验考察乙醇体积分数、溶剂用量、提取次数和时间对醇提工艺的影响。采用HPLC测定指标成分含量，流动相乙腈（A）-磷酸二氢铵溶液（B）梯度洗脱（0~10 min，10%A；10~25 min，10%~37%A；25~45 min，37%A），检测波长220 nm。 结果： 最佳提取工艺为加6倍量70%乙醇提取3次，每次2 h；延胡索乙素提取量332.04 mg，苦参碱和氧化苦参碱总量3 192.08 mg。 结论： 优选的提取工艺稳定可行，建立的色谱条件可较好地分离3个指标成分。

Optimization of Extraction Technology for Corydalis Rhizoma and Sophorae Flavescentis Radix in Liansong Yiboting Tablets by Orthogonal Design with Multi-indexes

Objective: To optimize extraction technology of Corydalis Rhizoma and Sophorae Flavescentis Radix in Liansong Yiboting tablets. Method: With extracting amounts of matrine,oxymatrine and tetrahydropalmatine as indexes,orthogonal test and single factor test was adopted to optimize extraction process by taking ethanol concentration,solid-liquid ratio,extracting times and time as factor.HPLC was employed to determine contents of index components with mobile phase of acetonitrile(A)-ammonium dihydrogen phosphate solution(B) for gradient elution(0-10 min,10%A;10-25 min,10%-37%A;25-45 min,37%A) and detection wavelength at 220 nm. Result: Optimum extraction technology was as following:extracted 3 times with 6 times the amount of 70% ethanol for 2 h each time.Extracting amount of tetrahydropalmatine was 332.04 mg,total extracting amount of matrine and oxymatrine was 3 192.08 mg. Conclusion: This optimized extraction technology was stable and feasible,these established chromatographic conditions had a good separation for three components.