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| 重组人促红细胞生成素对严重烫伤大鼠心肌细胞凋亡的影响 | |
| 引用本文： | 刘婷婷,蒋成,孙梦奎,周乾毅. 重组人促红细胞生成素对严重烫伤大鼠心肌细胞凋亡的影响[J]. 数理医药学杂志, 2010, 23(2): 151-153. DOI: 10.3969/j.issn.1004-4337.2010.02.010 |
| 作者姓名： | 刘婷婷 蒋成 孙梦奎 周乾毅 |
| 作者单位： | 武汉科技大学医学院组胚教研室,武汉,430065 |
| 基金项目： | 武汉科技大学大学生科技创新基金 |
| 摘 要： | 目的:研究重组人促红细胞生成素对Ⅲ°烫伤大鼠心肌凋亡的保护作用。方法:采用Ⅲ°烫伤模型,大鼠随机分成烫伤组、对照组、EPO治疗组。大鼠烫伤后按每kg体重1%烫伤面积4mL等渗盐水腹腔注射,并于伤后18h处死动物。HE染色及S-P免疫组织化学染色观察心肌细胞组织结构改变和bcl-2基因表达情况。结果:EPO治疗组大鼠心肌凋亡指数显著低于烫伤组(P<0.01),bcl-2蛋白表达也显著高于烫伤组(P<0.01)。结论:EPO对烫伤后大鼠心肌细胞凋亡有较好的抑制作用,其机制可能是EPO调控了凋亡相关基因bcl-2的表达。 |
| 关 键 词： | 促红细胞生成素 烫伤 细胞凋亡 心肌 |
Effects of rHuEPO on Myocardial Apoptosis in Severely Burned Rats |
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| Liu Tingting,et al. Effects of rHuEPO on Myocardial Apoptosis in Severely Burned Rats[J]. Journal of Mathematical Medicine, 2010, 23(2): 151-153. DOI: 10.3969/j.issn.1004-4337.2010.02.010 | |
| Authors: | Liu Tingting et al |
| Affiliation: | Liu Tingting,et al(Department of Histology , Embryology of Medical College,Wuhan University of Science , Technology,Wuhan 430065) |
| Abstract: | Objective.The myocardial apoptosis protective effect study of rHuEPO on burned Ⅲ degree of rat. Methods： Ⅲ degree burn model used, rats were randomly divided into burn group, the control group and treated group with EPO, Rats were killed after 18 h injury. The organizational structure changing of myocardial cells were observed by HE staining, The expression of bcl-2 gene in myocardial cells were observed by S-P immunohistochemical staining. Results： Myocardial apoptosis index in burn group with EPO treated was significantly lower than that of burn group, and the bcl-2 protein expression was significantly higher than that of burn group（P〈0.01）. Conclusions. EPO on cardiac myocyte apoptosis after burn injury has a better inhibitory effect,its mechanism may be the EPO gene regulation of apoptosis-related bcl-2 expression. |
| Keywords: | rHuEPO burn apoptosis myocardium |
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