电离辐射对作业人群外周血淋巴细胞DNA的损伤

目的　分别利用单细胞凝胶电泳技术 (SCGE)及染色体畸变试验观察电离辐射对作业人群外周血淋巴细胞DNA损伤作用和染色体损伤效应。方法　观察不同辐射剂量下外周血淋巴细胞DNA迁移长度及迁移率 ,同时观察不同剂量组下染色体畸变率及微核率。结果　各组染色体畸变率及微核率差异没有显著性 (P >0 0 5 ) ;接触射线的作业人群 ,彗星细胞率及DNA迁移长度明显超过对照组 ;同时 ,随着辐射剂量的加大 ,细胞DNA损伤级别亦有加重趋势。结论　SCGE技术可以检测电离辐射对作业人群的早期损害。

Study on DNA damage induced by ionizing radiation in workers'blood lymphocytes

Objective In order to investigate that the DNA damage and the frequency of chromosome aberration induced by ionizing radiation,the single cell gel electrophoresis(SCGE)and chromosome aberration test were carried out.Methods The length and rate of DNA migration of blood lymphocytes were analyzed in different dose exposed workers.The frequency of chromosome aberration and micronucleus were observed also.Results There was no significanty different both the frequency of chromosome aberration and micronucleus at the different doses(P>0 05).The significantly different length and rate of DNA migration were exhibited in varied ionizing radiation between the observed and control groups(P<0 05),and the level of DNA damage was increasing after exposed to more ionizing radiation.Conclusion These findings suggest that the technique of SCGE could be used to detect the earliest biological damage by ionizing radiation in exposed workers.