人组织激肽释放酶对周围神经系统损伤后的修复效应

目的:评价人组织激肽释放酶(HTK)促进周围神经系统损伤后的修复作用.方法:取30只体重180～200 g的雄性SD大鼠,随机分成3组:假手术组、HTK组(17.5×10~(-3) PNAU/kg)、对照组,每组各10只.术前第0天和术后第1、第3、第5、第7、第9、第11、第13天测定坐骨神经功能指数(SFI)以及静止坐骨神经指数(SSI),术后第14天测定坐骨神经干动作电位传导速度(NCV).结果:SFI、SSI值在假手术组手术前后未见明显改变.HTK组和对照组中,术后第1天SFI、SSI均为-100左右.以后发现HTK组的SFI、SSI恢复的情况要优于对照组,从第5天开始两组SFI、SSI值有统计学差异(P<0.05).术后第14天HTK组的NCV值要高于对照组,两组间有统计学差异(P<0.05).结论:HTK具有促进周围神经系统损伤后的修复作用.

Effect of human tissue kallikrein on peripheral nerve injury

AIM:To verify the therapeutic effection of human tissue kallikrein (HTK) on peripheral nervous system regeneration.METHODS:30 adult male SD rats weighted 180-200 g were randomly divided into 3 groups:Sham group,HTK group(17.5×10~(-3) PNAU/kg),Control group(n = 10).Calculating sciatic functional index (SFI) and static sciatic index (SSI) were performed on the day before nerve crushed and day 1,3,5,7,9,11,13 after operation in all rats.The electrophysiological results of the sciatic nerve was determined on the 14th day postoperation.RESULTS:There was no apparent functional deficit in the Sham group before and after operation.The HTK group showed a statistically significant improvement over the control group on the 5th day postoperation.The NCV of the HTK group was greater than the Control group (P<0.05).CONCLUSION:The treatment of HTK is effective on peripheral nervous system regeneration.