动态成像模式下蛋白A胶体金单分子三维构象原子力显微镜研究

Dynamic atomic force microscopic observation of the three-dimensional conformation of protein A-gold single molecule]

OBJECTIVE: To determine the three-dimensional (3D) conformation of staphylococcal protein A-gold (SPA-G) single molecule using atomic force microscope (AFM) so as to evaluate the feasibility of using this molecule for in situ labeling of the neuronal membrane protein. METHODS: AFM was used to acquire the images of SPA-G binding to the surface of mica under physiological condition for determining the 3D conformation of the molecule. RESULTS: SPA-G single molecule was shown to contain a characteristic structure with a chain in the shape of mirror image of the letter C, which had the dimension of 48.80 nm x 42.13 nm x 20.53 nm. CONCLUSION: AFM provide a new means for morphological investigation of the biomacromolecule at the nanometer scale in physiological conditions, and SPA-G can be utilized for in situ labeling of the neuronal membrane receptors.