Inhibitory effects of water extract of propolis on doxorubicin-induced somatic mutation and recombination in Drosophila melanogaster. |
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| Authors: | B L B Valadares U Graf M A Spanó |
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| Affiliation: | Instituto de Genética e Bioquímica, Universidade Federal de Uberlandia, Laboratório de Mutagênese, Umuarama, Uberlandia, MG, Brazil. |
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| Abstract: | ![]()
Propolis is a substance produced by honeybees (Apis mellifera L.). Its components are strong antioxidants and free radical scavengers. The aim of this study was to evaluate the protective effects of a water extract of Brazilian green propolis (WEP) combined with the antitumor agent doxorubicin (DXR) on Drosophila melanogaster wing cells through the somatic mutation and recombination test (SMART). Two different crosses were used: The standard (ST) cross and the high bioactivation (HB) cross. The HB cross is characterized by a constitutively enhanced level of cytochrome P450 which leads to an increased sensitivity to a number of promutagens and procarcinogens. Larvae obtained from these two crosses were chronically treated with different concentrations of WEP (12.5,25.0 and 50.0 mg/mL) alone or combined with DXR (0.125 mg/mL). The results obtained with the two different crosses were rather similar. Neither toxicity nor genotoxicity were observed in WEP treated series. Simultaneous treatment with different concentrations of WEP and DXR led to a reduction in the frequency of recombination compared to the treatment with DXR alone. This anti-recombinogenic effect was proportional to the concentrations applied, indicating a dose-response correlation and can be attributed to the powerful scavenger ability of WEP. |
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| Keywords: | 1-NP, 1-nitropyrene 2AF, 2-aminofluorene 3,4,5-triCQA, 3,4,5-tri-O-caffeoylquinic acid 3,4-diCQA, 3,4-di-O-caffeoylquinic acid 3,5-diCQA, 3,5-di-O-caffeoylquinic acid 4,5-diCQA, 4,5-di-O-caffeoylquinic acid 4-NO, 4-nitro-O-phenylenediamine 4-NQO, 4-nitroquinoline-N-oxide B[a]P, benzo(a)pyrene CA, caffeic acid CAS, chemical abstract service ChA, chlorogenic acid CHO, Chinese hamster ovary CP, cyclophosphamide D. melanogaster, Drosophila melanogaster DMC, daunomycin DNA, deoxyribonucleic acid DXR, doxorubicin ECD, 7-ethoxycoumarin-O-deethylase EEP, ethanolic extracts of propolis EROD, 7-ethoxyresorufin-O-deethylase flr, flare FM, mutation frequencies FR, recombination frequencies FT, frequencies of total spots GSH, glutathione HB, high bioactivation cross IQ, 2-amino-3-methylimidazo[4,5-f]quinoline MMC, mitomycin MN, micronucleus mwh, multiple wing hairs NADP, nicotinamide adenine dinucleotide phosphate NPD, 4-nitro-O- phenylenediamine RNA, ribonucleic acid S.cerevisiae, Saccharomyces cerevisiae SMART, somatic mutation and recombination test ST, standard cross WEP, water extract of propolis |
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