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| 体外培养口腔黏膜角化细胞生物学的特性 | |
| 引用本文： | 周海文,周曾同,商庆新,曹谊林. 体外培养口腔黏膜角化细胞生物学的特性[J]. 中国组织工程研究与临床康复, 2003, 7(11): 1628-1629 |
| 作者姓名： | 周海文 周曾同 商庆新 曹谊林 |
| 作者单位： | 1. 上海第二医科大学第九人民医院,口腔内科,上海市,200011 2. 上海第二医科大学第九人民医院,口腔研究所,上海市,200011 3. 上海第二医科大学组织工程研究中心,上海市组织工程重点实验室,上海市,200011 |
| 基金项目： | 国家自然基金(39970791)，上海市教委(99XD14003)~~ |
| 摘 要： | 目的一个稳定的人类正常口腔黏膜角化细胞体外培养体系对于研究组织工程化口腔黏膜构建及口腔黏膜上皮癌变及阻断有重要意义。研究体外培养的人正常口腔黏膜角化细胞的细胞生物学特性。方法通过测定细胞生长曲线及克隆形成率了解细胞生长基本规律及其增殖能力,并通过流式细胞仪检测细胞染色体倍性。结果细胞接种后第1～3天为静止生长期,第5～10天处于对数生长期,第12天后进入平台期。倍增时间为24～48h;接种密度为200/cm2时,培养细胞克隆形成率为0.979%。经流式细胞仪检测细胞染色体倍性结果表明原代及传代细胞均为二倍体细胞。结论体外连续培养的口腔黏膜角化细胞为正常上皮细胞,增殖能力良好。 |
| 关 键 词： | 细胞培养 黏膜 口腔 角蛋白细胞 |
| 文章编号： | 1671-5926(2003)11-1628-02 |
| 修稿时间： | 2002-11-08 |
The study of biologic specificities in culturing normal human oral keratinocytes in vitro |
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| Zhou Haiwen,Zhou Zengtong. The study of biologic specificities in culturing normal human oral keratinocytes in vitro[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2003, 7(11): 1628-1629 | |
| Authors: | Zhou Haiwen Zhou Zengtong |
| Affiliation: | Zhou Haiwen,Zhou Zengtong,Department of Oral Medicine,Ninth People's Hospital of Shanghai Second Medical University,Shanghai 200011 Shang Qingxin,Cao Yilin,Tissue Engineering Center of Shanghai Second Medical University,Tissue Engineering Key Laboratory of Shanghai,Shanghai 200011 |
| Abstract: | Aim To study biologic specificities in culturing normal human oral keratinocytes in vitro.Methods The growth curve, cloning efficiency and flow cytometry were used to measure basic law, proliferative ability and chromatosome ploidy of cells.Results After cells inoculation, 1-3 days was latent phase, 5-10 days was logarithmic phase and stagnate phase was from 12 days. The doubling time was 24-48 hours. The cloning efficiency was 0.979%at the inoculating density of 200 cells/cm2. The cultured cells were diploid cells.Conclusion The cultured cells are the normal cells and the state of proliferation is well. |
| Keywords: | cell culture mucous membrane mouth keratinocytes |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |