| RNA-DNA杂交法研究乳酸脱氢酶-C基因在睾丸中特异性表达 |
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| 引用本文： | 周孝瑚,李昌本,赵寿元.RNA-DNA杂交法研究乳酸脱氢酶-C基因在睾丸中特异性表达[J].复旦学报(医学版),1990(3). |
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| 作者姓名： | 周孝瑚 李昌本 赵寿元 |
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| 作者单位： | 上海医科大学基础医学部组织胚胎学教研室
(周孝瑚)，复旦大学遗传学研究所
(李昌本)，复旦大学遗传学研究所(赵寿元) |
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| 基金项目： | 复旦大学遗传工程开放实验室部分资助 |
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| 摘 要： | 用同位32~P标记乳酸脱氢酶-C(LDH-C)cDNA作为探针,与小鼠胸腺、脑、胰、心肌、骨骼肌、睾丸、肾、肺、肝的RNA以及人胰、皋丸、肝、骨骼肌、心肌及脑的RNA分别作点溃杂交(dot blot hybridization)及Northern印迹杂交,证实LDH-C基因只在睾丸中特异性表达。
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| 关 键 词： | 乳酸脱氢酶-C基因 基因表达 RNA-DNA杂交 |
IDENTIFICATION OF SPECIFIC EXPRESSION OF LDH-C GENE IN THE TESTIS BY RNA-DNA HYBRIDIZATION |
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| Zhou Xiaohu,Li Changben,Zhao Shouyuan.IDENTIFICATION OF SPECIFIC EXPRESSION OF LDH-C GENE IN THE TESTIS BY RNA-DNA HYBRIDIZATION[J].Fudan University Journal of Medical Sciences,1990(3). |
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| Authors: | Zhou Xiaohu Li Changben Zhao Shouyuan |
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| Abstract: | By using a CsCl gradient and ultraoentrifngation on a email scale, RNA was extracted from a variety of human and mouse tissues, including the thymus, brain, spleen, pancreas, testis, kidney, lung, liver and cardiac and skeletal muscles separately. 32P-labelled, nick-translated mouse LDH-C (laotate denydrogenase-C) cDNA was used as a probe. Dot and Nothern blot hydridizabion was carried out. Eesults showed that the expression of LDH-C gene is tissue-specific, in other words. LDH-C gene is specifically only expressed in the testis. |
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| Keywords: | lactate dehydrogenase-C gene gene expression ENA-DNA hybridization |
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