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Neutrophil extracellular traps and MPO in models of susceptibility and resistance against Entamoeba histolytica |
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| Authors: | Arturo Contis Montes de Oca Andrea Cruz Baquero Rafael Campos Rodríguez Luz María Cárdenas Jaramillo José Eduardo Aguayo Flores Saúl Rojas Hernández Alfonso Olivos García Judith Pacheco Yepez |
| Affiliation: | 1. Sección de Estudios de Posgrado e investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, CDMX, México;2. Sección de Estudios de Posgrado e investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, CDMX, México Bacteriología, Facultad de Ciencias de la Salud, Universidad Colegio Mayor de Cundinamarca, Bogotá, Colombia;3. Coordinación de Ciencias Morfológicas, Escuela Superior de Medicina, Instituto Politécnico Nacional, CDMX, México;4. Laboratorio de Inmunología Celular y Molecular, Sección de Estudios de Posgrado e investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, CDMX, México;5. Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, CDMX, México |
| Abstract: | The main effector mechanisms of neutrophils are the release of neutrophil extracellular traps (NETs) and myeloperoxidase (MPO). In this work, we evaluated the role of NETs and the activity of MPO in the interactions of rodent neutrophils with amoebae and in amoebic liver abscess (ALA)-resistant and ALA-susceptible models. We showed with in vitro assays that mice produced greater amounts of NETs and MPO than did hamsters, and the elastase activity was high in both models. However, the inhibition of NETs and MPO promoted an increase in amoeba viability in the mice. The mouse ALAs showed a more profound presence of NETs and MPO than did the hamster ALAs. We concluded that both effector mechanisms were essential for the amoebic damage and could prevent the formation of ALAs in the resistant model. |
| Keywords: | amoebic liver abscess BALB/c mice Entamoeba histolytica hamster myeloperoxidase neutrophils extracellular traps |