| 脊髓损伤局部表达NgR的巨噬细胞的体外分离培养 |
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| 引用本文： | 尹国栋,倪斌,周风金,杨军,郭翔,杨健,刘军.脊髓损伤局部表达NgR的巨噬细胞的体外分离培养[J].脊柱外科杂志,2009,7(4). |
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| 作者姓名： | 尹国栋 倪斌 周风金 杨军 郭翔 杨健 刘军 |
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| 作者单位： | 第二军医大学附属长征医院骨科,上海,200003 |
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| 摘 要： | 目的 从脊髓损伤局部分离培养巨噬细胞,并鉴定其表达Nogo受体(Nogo receptors, NgR)情况.方法 胰酶消化大鼠损伤脊髓组织获取其脊髓损伤局部巨噬细胞,细胞贴壁后用添加5%胎牛血清的RPMI 1640培养,显微镜下观察巨噬细胞形态及生物学特点,免疫荧光化学方法鉴定其是否表达CD68及NgR.结果 原代培养的巨噬细胞贴壁生长,细胞形态呈圆形、椭圆形等,可存活约3周,表现为CD68抗原阳性,多数细胞NgR抗原阳性.结论 成功地从脊髓损伤局部分离出巨噬细胞,多数细胞表达NgR抗原.
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| 关 键 词： | 脊髓损伤 巨噬细胞 细胞培养技术 荧光抗体技术 |
The separation and culture of macrophages expressed with NgR in vitro from spinal cord injury rats |
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| YIN Guodong,NI Bin,ZHOU Fengjin,YANG Jun,GUO Xiang,YANG Jian,LIU Jun.The separation and culture of macrophages expressed with NgR in vitro from spinal cord injury rats[J].Journal of Spinal Surgery,2009,7(4). |
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| Authors: | YIN Guodong NI Bin ZHOU Fengjin YANG Jun GUO Xiang YANG Jian LIU Jun |
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| Institution: | YIN Guodong,NI Bin,ZHOU Fengjin,YANG Jun,GUO Xiang,YANG Jian,LIU Jun.Department of Orthopaedics,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China |
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| Abstract: | Objective To separate and culture the macrophages expressed with NgR spinal cord injury rats in vitro.Methods Trypsin was applied to harvest the macrophages from the isolated traumatic spinal cord tissues.After adherence the macrophages were cultured in RPMI 1640 medium added with 5% fetal calf serum.The morphological and biological feature were observed in microscope.Immunochemistry staining was used to prove the characteristic of macrophages which expressed NgR and CD68.Results Most of the primary macroph... |
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| Keywords: | Spinal cord injury Macrophages Cell culture technique Flurescent antibody technique |
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