| RNA干扰技术沉默STAT3对人前列腺癌细胞生长的抑制作用 |
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| 引用本文： | 高丽芳,徐德启,邵月婷,赵丹,赵雪俭.RNA干扰技术沉默STAT3对人前列腺癌细胞生长的抑制作用[J].中华男科学杂志,2005,11(1):29-33,37. |
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| 作者姓名： | 高丽芳 徐德启 邵月婷 赵丹 赵雪俭 |
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| 作者单位： | 1. 吉林大学基础医学院病理生理学科,吉林大学前列腺疾病防治中心,吉林,长春,130021
2. Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research,Food and Drug Administration, Bethesda MD, 20892, USA |
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| 摘 要： | 目的: 探讨RNA干扰技术沉默STAT3基因表达对人前列腺癌细胞PC3及LNCaP的生长抑制作用。 方法: 针对STAT3mRNA序列设计合成 3对编码小干扰RNA(siRNA)的DNA模板,构建pSilencer1. 0 U6 siRNA STAT3重组质粒,转染PC3及LNCaP细胞;采用Western印迹、Northern印迹等技术观察重组质粒对STAT3基因表达的影响;用MTT法观察重组质粒对PC3及LNCaP细胞体外生长抑制作用;用流式细胞术(FCM)及吖啶橙染色检测重组质粒诱导细胞凋亡。 结果: 成功构建pSilencer1. 0 U6 siRNA STAT3重组质粒,并成功转染PC3及LNCaP细胞;Western印迹,Northern印迹结果证实重组质粒在mRNA及蛋白水平分别显著抑制STAT3基因表达,抑制率为60% ~75%;MTT及FCM结果证明上述重组质粒可显著抑制PC3及LNCaP细胞的体外生长并诱导PC3细胞凋亡。结论: pSilencer1. 0 U6 siRNA STAT3可抑制STAT3在人前列腺癌细胞中的表达,并抑制肿瘤细胞的生长,促进其凋亡。
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| 关 键 词： | 前列腺癌 RNA干扰 STAT3 凋亡 |
| 文章编号： | 1009-3591(2005)01-0029-06 |
Knockdown of STAT3 Expression Using siRNA Inhibits the Growth of Prostate Cancer Cell Lines |
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| GAO Li-fang ,XU De-qi ,SHAO Yue-ting ,ZHAO Dan ,ZHAO Xue-jian.Knockdown of STAT3 Expression Using siRNA Inhibits the Growth of Prostate Cancer Cell Lines[J].National Journal of Andrology,2005,11(1):29-33,37. |
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| Authors: | GAO Li-fang XU De-qi SHAO Yue-ting ZHAO Dan ZHAO Xue-jian |
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| Institution: | Department of Pathophysiology, School of Basic Medicine, Jilin University, Changchun, Jilin 130021, China. |
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| Abstract: | Objective: To study the effects of pSilencer 1.0-U6-siRNA-STAT3 on the growth of PC3 and LNCaP cells. Methods: Three pairs of DNA template coding siRNA were synthesized against STAT3 to reconstruct pSilencer 1.0-U6-STAT3-siRNA, which was transfected into PC3 and LNCaP cells. The STAT3 expression in PC3 cells and LNCaP were transfected with pSilencer 1.0-U6-siRNA-STAT3, and it was detected by Western blot and Northern blot. MTT and FCM were used to observe the growth-inhibiting ratio and apoptosis in PC3 cells. Results: Western blot and Northern blot analyses demonstrated that pSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit the expression of STAT3 in PC3 and LNCaP cells; MTT and FCM results showed that it could suppress the growth of PC3 cells and LNCaP and induce apoptosis of PC3 cells in vitro. Conclusion: PSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit STAT3 expression, suppress the growth of PC3 and LNCaP cells and induce the apoptosis of PC3 cells. Natl J |
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| Keywords: | STAT3 |
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