毛细管气相色谱法测定伤痛宁膏中薄荷脑和水杨酸甲酯的含量

目的建立用气相色谱法同时测定伤痛宁膏中薄荷脑和水杨酸甲酯含量的方法。方法以正十五烷为内标物,DB-WAX弹性石英毛细管柱(30m×0.32mm,0.25μm),FID检测器,氮气为载气,柱温为130℃,检测器温度为200℃,分流比为12∶1。结果共测定了6批样品,在该色谱条件下,薄荷脑和水杨酸甲酯及内标物正十五烷均得到良好的分离。薄荷脑和水杨酸甲酯的加样回收率分别为101.52%(RSD=2.66%)、100.34%(RSD=3.38%)。结论本法灵敏、准确,重复性好,可用于控制该制剂的质量。

Determination of Menthol and Methyl Salicylate in Shangtongning Adhesive Plaster by Capillary Gas Chromatography

Objective To establish a capillary gas chromatography method for the determination of menthol and methyl salicylate in Shangtongning adhesive plaster. Methods The internal standard substance of n-Pentadecane was employed. The GC system consisted of a DB-WAX capillary column （30 m × 0.32 mm, 0.25 μm）, nitrogen as the carrier gas, column temperature at 130 ℃, FID as the detector at 200 ℃, and with a split ratio of 12 ： 1. Results The contents of menthol and methyl salicylate in 6 lots of samples were determined and the constituents concerned were separated well under the chromatographic condition. The average recovery of menthol and methyl salicylate were 101.52% （RSD=2.66%） and 100.34% （RSD= 3.38%）, respectively. Conclusion The method was sensitive, accurate, specific and suitable for the quality control of the preparation.