HBV-DNA与其病毒免疫标志物的关系

目的:探讨乙型肝炎病毒(HBV)感染者HBV-DNA与病毒免疫标志物的关系。方法:采用实时荧光定量PCR(FQ-PCR)对568份HBV感染者血清中HBV-DNA含量进行检测,同时用ELISA法检测其HBV免疫标志物。结果:HBsAg、HBeAg和HBcAb阳性组HBV-DNA阳性率约为98.9%;HBsAg、HBcAb和HBeAb阳性组HBV-DNA阳性率约为65.4%;HBsAb、HBcAb和HBeAb阳性组HBV-DNA阳性率约为13.9%;三组间HBV-DNA阳性率有明显差异(P<0.01)。HBeAg阳性标本中HBV-DNA阳性率最高,达92.0%;HBsAg与HBV-DNA的符合率最高,为77.6%。结论:检测HBV-DNA含量结合血清病毒标志物对乙型肝炎的临床诊断治疗以及病情判断有重要意义。

Relationship between HBV-DNA and HBV Immunological Markers in Patients Infected HBV

Objective To investigate the relationship between HBV-DNA and HBV Immunological Markers in patients who infected HBV.Methods FQ-PCR was used to detect the contents of serum HBV-DNA in 568 patients infected HBV and ELISA was used to detect the serum HBV Immunological Markers.Results The positive rate of HBV-DNA in HBsAg,HBeAg and HBcAb positive samples was 98.9%.In HBsAg,HBcAb and HBeAb positive samples it was 65.4% and in HBcAb,HBeAb and HBsAb positive samples it was 13.9%.There was significant difference among the three groups(P<0.01).The positive rate of HBV-DNA in HBeAg positive samples and the coincidence of HBV-DNA and HBsAg was the highest,it was 92.0% and 77.6% respectively.Conclusion It was very important to detect the contents of serum HBV-DNA and the Immunological Markers for the clinic diagnosis and therapy and the judgement of pathogenetic condition in patients with chronic hepatitis type B.