雌激素受体基因多态性与急性心肌梗死关系的研究

目的 了解雌激素受体（ER）基因多态性在中国汉族人群中的分布及其与急性心肌梗死（AMI）是否相关。方法 应用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析方法，检测75例AMI患者和118例正常对照者ER基因型，结合血脂水平、选择性冠状动脉造影（SCA）结果探讨两者间的关系。结果 ER等位基因X、x和P、p频率在AMI组和对照组分别为0.207、0.793，0．169、0.831;0.287,0.713,0.339,0.661。基因型频率分布符合Hardyweinberg平衡定律。XbaI酶切多态性基因型频率，等位基因频率及结合XbaI和PvuⅡ两个酶切多态性分析在组内、组间比较差异均无显著意义（P＞0．05），且ER基因型间血脂水平、SCA结果在组内比较差异均无显著意义（P＞0．05）。但是，PvuⅡ酶切多态性在正常对照组与AMI组比较差异有显著意义（P＜0．05），而Pvu Ⅱ酶切多态性与AMI有相关性（P＜0．05），Pvu Ⅱ基因多态性可能是AMI发病的危险因素之一。

Association between estrogen receptor gene polymorphisms and acute myocardial infraction

Objective To study the distribution of estrogen receptor (ER) in Chinese Hans population and the association of ER gene polymorphisms with acute myocardial infarction (AMI) Methods ER genotyping was performed in 75 AMI patients and 118 controls by PCR RFLP The serum lipid levels of the subjects were also determined 46 AMI patients were examined by selected coronary angiography (SCA) Results ER allelic frequencies of X,x and P,p alleles were 0 207,0 793;0 169,0 831 and 0 287,0 713; 0 399, 0 661 in AMI and control groups respectively There was no significant difference between the distribution of Xbal and PvaII polymorphisms in AMI group and control gorup ( P >0 05) The serum lipid levels and the results of SCA were also not significantly difference among genotypes in intra group But the distributions of PvuII genotypes were significantly different between AMI group and controls ( P <0 05) Conclusion XbaI polymorphisms are not related to AMI But PvuII polymorphisms are associated with AMI, they might be a risk factor for AMI