不同程度、类型脊髓损伤后活性氧水平的变化

目的研究脊髓损伤（SCI）后脊髓组织内丙二醛（MDA）和超氧化物岐化酶（SOD）的含量变化，以探讨活性氧（ROS）在脊髓继发性损伤中的作用机制．方法30只健康雌性成年SD大鼠采用改良式Allen氏法和脊髓横截面切割法分别造成50cmg、25cmg挫伤和半、全横截面切割伤四组及对照组模型。测定造模后6h损伤组和对照组脊髓组织内MDA和SOD含量并各组之间相互作比较．结果SCI后6h，四种损伤组与对照组比较MDA含量都明显升高（P〈0．05），SOD活性都显著下降（P〈0．05）；在同一类型SCI的不同程度之间比较除半、全横截面切割伤之间SOD活性差别显著（P〈0．05）外，其余都显示无显著性的差异（P〉0．05）．结论不同程度和类型的SCI后脊髓组织内ROS水平明显增高，因而说明在脊髓继发性损伤的病理过程中ROS可能是一种重要的参与者；同一类型的SCI脊髓组织的ROS水平在不同程度损伤模型之间比较，ROS含量与损伤程度并非完全呈正相关．

Changes of ROS Level in Different Types and Degrees of Spinal Cord Injury

Objective To observe the changes of malonyldialdehyed(MDA) and superoxide dissmutas(SOD) contents in spinal cord after two different types and degrees of spinal cord injury(SCI) and to study the reactive oxygen species variation in secondary pathological damage of the spinal cord.Methods Thirty Sprague-Dawley rats were randomly divided into five groups: control group,50cmg and 25cmg contusion groups,hemi-section and transection injury groups.The SCI models in rats were made with modified Allen's method and intersection cutting.The amount of MDA and SOD of spinal cord tissue were determined in 6h after injury.Results The concentration of MDA significantly increased and the activity of SOD significantly descended in all the types of SCI in 6h(P<0.05).No statistically significant difference was seen regarding MDA and SOD contents in the groups of the same type of SCI(P>0.05) except the SOD in the hemi-section and transection injury groups(P>0.05).Conclusions The reactive oxygen species(ROS) plays a crucial role in the secondary pathological damage of SCI.The level of ROS in the injured spinal cord tissue is not completely consistent with degree of injury.