| 地塞米松对顺铂诱导的HepG2细胞凋亡及Bcl-2和Caspase-3蛋白表达的影响 |
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| 引用本文： | 谭俊,阮冰,周密,高国生. 地塞米松对顺铂诱导的HepG2细胞凋亡及Bcl-2和Caspase-3蛋白表达的影响[J]. 中国临床药理学与治疗学, 2012, 17(10): 1118-1123 |
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| 作者姓名： | 谭俊 阮冰 周密 高国生 |
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| 作者单位： | [1]浙江大学医学院附属第一医院感染性疾病诊治国家重点实验室,浙江杭州310003 [2]浙江宁波市第二医院肝病科,浙江宁波315016 [3]宁波大学医学院微免教研室,浙江宁波315000 |
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| 基金项目： | 浙江省医药卫生一般研究计划（2011KYB107） |
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| 摘 要： | 目的:探讨地塞米松对顺铂诱导HepG2细胞凋亡的影响,并观察Bcl-2和Caspase-3蛋白的表达。方法:HepG2细胞与顺铂及不同浓度的地塞米松共培养,RT-PCR检测Bcl-2、Caspase-3的mRNA表达,Western blot检测Bcl-2、Caspase-3蛋白表达,流式细胞术(FACS)检测各组HepG2细胞的凋亡情况。结果:随着地塞米松浓度的增加,HepG2细胞中Bcl-2蛋白表达量升高,Caspase-3蛋白表达量下降。同时细胞凋亡率降低。结论:地塞米松部分通过Bcl-2途径抑制肝癌细胞的凋亡,而Caspase-3又在调控细胞凋亡中起重要作用。
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| 关 键 词： | 地塞米松 顺铂 HepG2细胞 凋亡 |
Effects of Dexamethasoneon apoptosis of HepG2 cells induced by Cisplatin and expression of Bcl-2 and Caspase-3 |
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| TAN Jun,RUAN Bing,ZHOU Mi,GAO Guo-sheng. Effects of Dexamethasoneon apoptosis of HepG2 cells induced by Cisplatin and expression of Bcl-2 and Caspase-3[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(10): 1118-1123 |
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| Authors: | TAN Jun RUAN Bing ZHOU Mi GAO Guo-sheng |
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| Affiliation: | 1 Department of Infectious Diseases,National Key Laboratory for Diagnosis and Treatment of Infectious Diseases in China,the First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang,China;2 Department of Liver Diseases,Ningbo No.2 Hospital,Ningbo 315016,Zhejiang,China;3 Department of Microbiology and Immunology,Medical College,Ningbo University,Ningbo 315211,Zhejiang,China |
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| Abstract: | AIM： To investigate the mechanism of Dexamethasone（DEX） on apoptosis of HepG2 cells induced by Cisplatin（DDP） and the expression of Bcl-2 and Caspase-3.METHODS： HepG2 and DDP were co-cultured,a serials of concentrations DEX were added into it,the expression of mRNA and protein of Bcl-2 and Capase-3 were analyzed separately by RT-PCR and Western blot,and the percentage of cell apoptosis was analyzed by FACS.RESULTS：With DEX increases in concentration,the expression of Bcl-2 was increased and expression of Caspase-3 was decreased correspondently,the rate of apoptosis of HepG2 descends clearly.CONCLUSION： DEX restrains apoptosis of HepG2 cells induced by Cisplatin（DDP） partly through Bcl-2 anti-apoptosis pathways,and Caspase-3 plays an important role in regulating the cell apoptosis. |
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| Keywords: | Dexamethasone Cisplatin HepG2 cells Apoptosis |
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