| 人血浆中茶碱和多索茶碱的HPLC法测定 |
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| 引用本文： | 刘奕芳,沈维敏,沈杰,宋钟娟,夏云.人血浆中茶碱和多索茶碱的HPLC法测定[J].中国医药工业杂志,2010,41(2). |
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| 作者姓名： | 刘奕芳 沈维敏 沈杰 宋钟娟 夏云 |
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| 作者单位： | 1. 复旦大学附属华东医院药剂科,上海,200040
2. 复旦大学附属华东医院呼吸内科,上海,200040 |
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| 摘 要： | 建立了HPLC法测定人血浆中的茶碱和多索茶碱.采用C_(18)色谱柱,以咖啡因为内标,甲醇-水(23:77)为流动相,检测波长273 nm.血浆经甲醇沉淀蛋白后直接进样.茶碱和多索茶碱在2.5～40μg/ml浓度范围内线性关系良好,日内、日间RSD均小于5.2%.
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| 关 键 词： | 茶碱 多索茶碱 高效液相色谱 测定 |
Determination of Theophylline and Doxofylline in Human Plasma by HPLC |
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| Authors: | LIU Yifang SHEN Weimin SHEN Jie SONG Zhongjuan XIA Yun |
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| Abstract: | An HPLC method was established for the determination of theophyUine and doxofyline in human plasma.A C_(18) column was used,with caffeine as internal standard and the mobile phase of methanol-water(23:77)at 273 nm.The plasma samples were injcoted directly after precipitation by methan01.The calibration curves of theophylline and doxofylline were linear in the concentration range of 2.5-40μg/ml.The intra-and inter-day RSDs WgTe less than 5.2%. |
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| Keywords: | theophylline doxofylline HPLC determination |
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