精索静脉曲张严重少精子症患者Y染色体微缺失分析

目的:探讨精索静脉曲张和Y染色体微缺失对生精障碍的影响。方法:对随机挑选的100例左侧精索静脉曲张严重少精子症患者(精子浓度<5×106/ml,组1),100例左侧精索静脉曲张轻度少精子症患者精子浓度(10～20)×106/ml,组2],100例特发性严重少精子症患者(组3),100例特发性轻度少精子症患者(组4)和30例正常生育男性对照组(组5)采用聚合酶链反应(PCR)技术进行Y染色体微缺失检测,选取无精子因子(AZF)a、b和c区9个序列标签位点(STS)。结果:组1中有19例患者存在AZF微缺失(19%);组3中有11例患者存在AZF微缺失(11%),其余各组均未发现AZF微缺失;组1比组3有较高的缺失率。结论:在治疗精索静脉曲张严重生精障碍患者前应先进行Y染色体微缺失检测,避免不必要的治疗。

Y chromosome microdeletions in severe oligospermia men with varicocele

Objective: To investigate Y chromosome microdeletions in severe oligospermia men with varicocele.Methods: We randomly selected 100 cases of severe oligospermia with left varicocele(sperm concentration<5×106/ml,group 1),100 cases of mild oligospermia with left varicocele(sperm concentration 10-20×106/ml,group 2),100 cases of idiopathic infertility with severe oligospermia(group 3),100 cases of idiopathic infertility with moderate oligospermia(group 4) and 30 normal fertile men as controls(group 5).We used polymerase chain reaction(PCR) technology to screen 9 sequence tagged sites(STS) of the AZF a,b and c regions and detect Y chromosome microdeletions.Results: AZF microdeletions were found in 19 patients in group 1(19%) and 11 in group 3(11%),with a higher rate in the former than in the latter,but not in the other three groups.Conclusion: Screening of Y chromosome microdeletions should be performed before the treatment of severe spermatogenesis with varicocele.