Simple in vitro migration assay for neural crest cells and the opposite effects of all‐trans‐retinoic acid on cephalic‐ and trunk‐derived cells |
| |
| Authors: | Makoto Usami Katsuyoshi Mitsunaga Tomohiko Irie Atsuko Miyajima Osamu Doi |
| |
| Affiliation: | 1. Division of Pharmacology, National Institute of Health Sciences, , Tokyo, Japan;2. School of Pharmaceutical Sciences, Toho University, , Chiba, Japan;3. Division of Medical Devices, National Institute of Health Sciences, , Tokyo, Japan;4. Laboratory of Animal Reproduction, United Graduate School of Agricultural Science, Gifu University, , Gifu, Japan |
| |
| Abstract: | ![]()
Here, we describe a simple in vitro neural crest cell (NCC) migration assay and the effects of all‐trans‐retinoic acid (RA) on NCCs. Neural tubes excised from the rhombencephalic or trunk region of day 10.5 rat embryos were cultured for 48 h to allow emigration and migration of NCCs. Migration of NCCs was measured as the change in the radius (radius ratio) calculated from the circular spread of NCCs between 24 and 48 h of culture. RA was added to the culture medium after 24 h at embryotoxic concentrations determined by rat whole embryo culture. RA (10 μM) reduced the migration of cephalic NCCs, whereas it enhanced the migration of trunk NCCs, indicating that RA has opposite effects on these two types of NCCs. |
| |
| Keywords: | developmental toxicity embryo migration assay neural crest cell rat |
|
|
