膈下逐瘀汤对免疫性肝纤维化大鼠肝组织MMP-9和TIMP-2表达的影响

目的:观察膈下逐瘀汤对肝纤维化大鼠肝组织基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶组织抑制因子-2(TIMP-2)表达的影响。方法:大鼠随机分为空白对照组、膈下逐瘀汤组、猪血清组、猪血清+膈下逐瘀汤组。猪血清腹腔注射诱导大鼠免疫性肝纤维化模型,同时膈下逐瘀汤灌胃给药干预。消化法检测肝组织羟脯氨酸含量,ELISA法检测肝组织MMP-9和TIMP-2蛋白含量,实时定量RT-PCR检测肝组织MMP-9和TIMP-2 mRNA表达。结果:与模型对照组比较,膈下逐瘀汤可显著降低肝组织羟脯氨酸、MMP-9及TIMP-2蛋白含量,下调肝组织MMP-9、TIMP-2 mRNA水平。结论:膈下逐瘀汤可有效地逆转实验性大鼠肝纤维化,其机制可能与调节MMP-9和TIMP-2的表达有关。

Effects of Gexiazhuyu Decoction on the expression of MMP-9 and TIMP-2 in rats with liver fibrosis

Objective: To study the effects of Gexiazhuyu decoction on established rat liver fibrosis,and examine its effects on the expression of matrix metalloproteinase-9(MMP-9) and tissue inhibitors of metalloproteinase-2(TIMP-2).Methods: Sixty male Sprague-Dawley rats were randomly divided into four groups as follows: blank control group,Gexiazhuyu decoction group,porcine serum group,porcine serum plus Gexiazhuyu decoction group.All rats were sacrificed at the end of experiment,and liver were collected for analysis.The hydroxyproline(Hyp) content in liver tissue was detected by spectrophotography.The content of MMP-9 and TIMP-2 in the liver was detected by enzyme linked immunosorbent assay.MMP-9 and TIMP-2 mRNA levels in the liver was detected by real-time reverse transcriptase polymerase chain reaction.Results: Compared with the porcine serum group,Hyp content in liver tissue of rats given Gexiazhuyu decoction decreased significantly.mRNA expressions and protein content of MMP-9 were elevated significantly,and TIMP-2 content in liver tissue was decreased significantly in porcine serum plus Gexiazhuyu decoction group.Conclusion: Gexiazhuyu decoction may exert anti-hepatic fibrosis effect in rats via regulation of MMP-9 and TIMP-2 gene expression.