| 小鼠肝癌细胞系Hepa1-6 B7.1瘤苗抗肿瘤免疫的实验研究 |
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| 引用本文： | 杨欣伟,隋延仿,李增山,曲萍,武文,叶菁,董海龙,张秀敏. 小鼠肝癌细胞系Hepa1-6 B7.1瘤苗抗肿瘤免疫的实验研究[J]. 医学争鸣, 2003, 24(6): 502-505 |
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| 作者姓名： | 杨欣伟 隋延仿 李增山 曲萍 武文 叶菁 董海龙 张秀敏 |
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| 作者单位： | 第四军医大学基础部病理学教研室,陕西,西安,710033;第四军医大学基础部病理学教研室,陕西,西安,710033;第四军医大学基础部病理学教研室,陕西,西安,710033;第四军医大学基础部病理学教研室,陕西,西安,710033;第四军医大学基础部病理学教研室,陕西,西安,710033;第四军医大学基础部病理学教研室,陕西,西安,710033;第四军医大学基础部病理学教研室,陕西,西安,710033;第四军医大学基础部病理学教研室,陕西,西安,710033 |
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| 基金项目： | 国家自然科学基金,39770827, |
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| 摘 要： | 目的：研究小鼠肝癌细胞系Hepal-6转染B7.1基因后在体内外抗肿瘤免疫的作用。方法：采用脂质体介导的方法用pLXSNB7.1转染PA317包装细胞,经G418筛选出高滴度阳性的细胞克隆,以流式细胞仪检测B7.1分子的表达情况;用所获病毒上清进一步感染Hepal-6细胞,观察转染B7.1基因后Hepal-6细胞作为肿瘤疫苗在体内外诱发抗肿瘤免疫的情况。结果：Hepal-6/B7.1高表达B7.1分子,其致瘤性明显降低。接种小鼠后成瘤率为20%,而亲代Hepal-6细胞成瘤率为100%,转B7.1基因肿瘤细胞疫苗可抑制肿瘤生长速度,延长荷瘤小鼠生存期;且转B7.1基因肿瘤细胞疫苗具有保护性免疫反应。结论：转B7.1基因的肿瘤细胞作为肿瘤疫苗可有效地激发机体的抗肿瘤免疫反应。
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| 关 键 词： | 小鼠 癌 肝细胞 B7.1 癌症疫苗 |
| 文章编号： | 1000-2790(2003)06-0502-04 |
| 修稿时间： | 2002-10-15 |
Experimental study of anti-tumor immunity induced by tumor cells vaccine of murine hepatocellular carcinoma cell line (Hepa1-6) |
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| Abstract: | AIM: To study the efficacy of anti tumor immunity both in vivo and in vitro with B7.1 gene induced into murine hepa1 6 hepatocellular carcinoma cells. METHODS : pLXSN B7.1 was transfected into packaging cell line PA317 by liposome induction and high titer positive clone was acquired by G418. The expression of B7.1 molecules was detected by FACS. Anti tumor immunity in vivo and in vitro was observed following transfection of B7.1 gene into Hepa 1 6 cell line, which acted as a tumor vaccine. RESULTS: B7.1 molecules were highly expressed in Hepa 1 6/B7.1, with decreased tumor induction and protective immunity effect. CONCLUSION: Hepa1 6 hepatocellular carcinoma cells transfected with B7.1 genes as a kind of tumor vaccine can effectively trigger the host anti tumor immunity. |
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| Keywords: | mice carcinoma hepatocellular B7.1 cancer vaccines. |
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