人牙周膜成纤维细胞增殖与压力关系的初步观察 |
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引用本文: | 张惠,施生根,宋应亮,刘新平,王吉村,党平. 人牙周膜成纤维细胞增殖与压力关系的初步观察[J]. 实用口腔医学杂志, 2001, 17(2): 130-133 |
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作者姓名: | 张惠 施生根 宋应亮 刘新平 王吉村 党平 |
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作者单位: | 1. 西安第四军医大学口腔医学院修复科 2. 第四军医大学生物化学与分子生物学教研室 |
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摘 要: | 目的:在体外培养环境下观察压力对人牙周膜成纤维细胞(HPLF)增殖的影响。方法:取第4-6代培养的HPLF,应用可控压力细胞加载装置分别间断性施加30、60、90kPa的压力,分别在培养3、5、7d后用MTT法测定各组的A值。结果:HPLF在30、60、90kPa的压力培养环境下MTT反应的A值显著小于对照组,压力值越大,A值越小。结论:30、60、90kPa间断性压力显著抑制PLF细胞增殖,该抑制作用随压力值增大而增强。
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关 键 词: | 牙周韧带 成纤维细胞 细胞增殖 压力 牙周膜 |
文章编号: | 1001-3733(2001)-02-0130-04 |
修稿时间: | 2000-09-15 |
The effects of pressure on proliferation of human periodontal ligament fibroblasts |
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Abstract: | Objective:To investigate the effects of pressure on the proliferation of human periodontal ligament fibroblasts(HPLFs).Method:In vitro cultured HPLFs of passage 4~6 were incubated under discontinuous pressure of 30,60 and 90 kPa for 3,5 and 7 d respectively.Cell proliferation was studied with MTT assay. Results:On day 3,5 and 7 the A values of HPLFs cultured under pressure were smaller than those of the controls (P<0.01);the A values decreased with the increace of pressure (P<0.05). Conclusion: Discontinuous pressure can significantly suppress HPLF proliferation in a pressure value dependent way. |
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Keywords: | Periodontal ligament Fibroblast Proliferation Pressure |
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