血浆对臭氧在水介质中杀菌效果影响的研究

目的探索血浆对臭氧在水介质中杀菌作用的干扰效应，为医用臭氧对腹水等体液中细菌杀灭作用研究摸索臭氧作用的浓度及时间参数。方法在含不同浓度血浆的生理盐水中加入大肠埃希菌（ATCC25922）和金黄色葡萄球菌（ATCC25923），悬液定量法计算高浓度臭氧（90μg／mL）对其杀菌率；臭氧作用于含10．0％血浆的生理盐水．摸索臭氧有效杀菌的浓度和作用时间参数。结果臭氧杀菌效果随生理盐水中血浆浓度的提高而降低，血浆含量大于10．0％后杀菌效果显著降低（P〈0．05），90μg／mL臭氧作用20min（流速12ml／min）对含10．0％血浆的生理盐水中大肠埃希菌、金黄色葡萄球菌杀菌率可分别达到100％、99．05％。不同浓度组（40μg／mL、60μg／mL、90μg／mL）的臭氧杀菌效果有显著性差异（P〈0．05），臭氧作用不同时间（5min、10min、20min）杀菌效果亦有显著性差异（P〈0．05），以浓度为90μg／mL臭氧作用20min杀菌效果最好。结论血浆可干扰臭氧在生理盐水中的杀菌作用．血浆含量在10．0％以下时影响作用较小，提高臭氧浓度、延长作用时间可提高杀菌率。

Influence of Plasma on the Efficacy of Ozone in Aqueous Medium

Objective To study the influence of plasma on the sterilization effect of ozone in aqueous medium and to provide data for further evaluation on the efficacy of ozone for sterilization of body fluid such as ascites. Method Escherichia coli（ATCC25922）and Staphylococcus areus（ATCC25923）were exposed to various concentrations of normal saline diluted plasma. The killing rate of ozone （90 μg/mL） was calculated. The dose of ozone and the kinetic of bacterial cell killing were determined in normal saline with 10.0% human plasma. Result The efficacy of ozone in normal saline was dose dependently decreased, especially at the plasma concentration of 10% （P〈0.05）. Under the conditions of 10% plasma, 90 μg/mL and the flow rate of 12 ml/min, the killing rate for Escherichia coli and Staphylococcus aureus was 100% and 99.05%, respectively. Differences were observed in groups treated with 40 μg/mL, 60 μg/mL and 90 μg/mL of ozone （P〈0.05）. The efficacy was also varied with the exposure time （5 min, 10 min and 20 min）. The best bactericidal effect was observed when the cells were treated with 90 μg/mL of ozone and the exposure time was 20 min. Conclusion The efficacy of ozone was reduced at the plasma concentration of 10%. The efficacy can be improved by increasing both the concentration and the exposure time to ozone.