| 利用原生质体诱变筛选L-异亮氨酸高产菌株 |
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| 引用本文： | 刘党生,李福德,韩杰.利用原生质体诱变筛选L-异亮氨酸高产菌株[J].沈阳药科大学学报,1990(1). |
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| 作者姓名： | 刘党生 李福德 韩杰 |
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| 作者单位： | 沈阳药学院微生物研究室，沈阳药学院微生物研究室，沈阳药学院微生物研究室 本院51期微生物制药专业学生 |
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| 摘 要： | 在捧杆菌原生质体制备中,以单一甘氨酸代替青霉素与溶菌酶进行细胞脱壁处理,以含0.3M蔗糖的普通肉汤培养基作为再生培养基。L-异亮氨酸产生菌A6在肉汤中培养至对数期,加入0.3M蔗糖和3%甘氨酸,继续培养8h,原生质体的形成率达98%。原生质体经洗涤后的再生率为81.6%,不经离心洗涤直接涂皿的再生率为81.2%。A6菌株的原生质体经紫外线诱变处理后,再生菌株的L-异亮氨酸产量高达14.3mg/ml,比原菌株产量提高30%左右。
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| 关 键 词： | L-异亮氨酸 甘氨酸 原生质体 再生 |
SCREENING FOR L-ISOLEUCINE PRODUCER BY MUTAGENIZING PROTOPLAST PREPARED BY IMPROVED METHOD AND REGENERATION |
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| Liu Dangsheng,Li Fude,Han Jie,Research Laboratory of Microbiology.SCREENING FOR L-ISOLEUCINE PRODUCER BY MUTAGENIZING PROTOPLAST PREPARED BY IMPROVED METHOD AND REGENERATION[J].Journal of Shenyang Pharmaceutical University,1990(1). |
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| Authors: | Liu Dangsheng Li Fude Han Jie Research Laboratory of Microbiology |
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| Abstract: | In the preparation of Corynebacterium protoplast, glycine is used instead of
penicillin and lysozyme. Common broth medium containing 0.3M sucrose is used
for regeneration. Strain A6, a L-isoleucine producer, is inoculated in broth and is
cultivated to logarithmic phase, then 0.3M sucrose and 3% glycine are added to it.
After cultivation continues for 8 hour% the rate of protoplast formation is up to
98%.The regeneration rate of protoplast washed is 81.6% while that of protoplast
not washed is 86.2%.The protoplast of strain A6 is treated by UV and the strain
from regeneration can produce L-isoleucine up to 14.3mg/ml, 30% more than the
initial. |
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| Keywords: | L-isoleucine L-glycine Protoplast Regeneration |
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