乙酰胆碱酯酶在家兔小脑内的分布

研究了11只家兔小脑内AChE活性的分布。采用改良的经典组织化学技术来显示AChE活性。小脑皮质颗粒层一般有很强的AChE活性,而浦肯野细胞层的分子层的浅部则呈AChE反应阴性。分子层的深部和小脑白质的AChE活性较弱。而且有很多阳性神经元出现在后叶的分子层中,以X小叶最为密集。在小脑白质中,发现5条与正中面相平行的AChE活性较强的纵带。其中蚓状正中面上的正中带出现在除Ⅵ、Ⅶ小叶以外的各小叶中;对称分布的内侧带出现在前叶的Ⅱ到Ⅴ小叶和后叶的Ⅷ、Ⅸ小叶;外侧带只出现在Ⅸc和Ⅸd分叶。小脑中央核显示很强的AChE活性。在颗粒层的胶质细胞和白质中发现有丁酰胆碱酯酶(BuChE)的活性,而且在近颗粒层的白质活性更强。

AChE Distribution in the Cerebellum of Rabbit

The distribution of AChE activity in the cerebellum of the rabbit was studied in 11 ani-mals. A modified classical histochemical technique was used for showing the activity of AChE in frontal, sagittal and horizontal sections of the cerebellum. In the cerebellar cortex, the granular layer was generally shown to have rather strong activity of AChE while the Purkinje cell’s layer and the superficial part of the molecular layer were negative for AChE reaction. Like those in the white matter, the activity of AChE in the deep part of the mole-cular layer was rather weak. In addition, there were many AChE-positive neurons in the molecular layer of the posterior lobe, most of them were concentrated in the lobule X. In the white matter of the cerebellum, there were 5 longitudinal zones showning relatively strong activity of AChE paralleled to the midplane. Among them, the intermedian zone on the mid-plane of the vermis could be seen in all lobules except lobules Ⅵ and Ⅶ. The medial zones were symmetrically located in the lobules Ⅱ~Ⅴof anterior lobe and lobules Ⅷ, and the lateral zones only in the sublobules IXc and IXd of the posterior lobe, Also, a strong AChE activity was found in the cerebellar nuclei. The activity of BuChE was demonstrated in the glial cells of the granular layer and the white matter in which a little stronger reaction of BuChE were seen near the granular layer.