2-脱氧-D-葡萄糖对人脐静脉内皮细胞的作用

目的观察2-脱氧-D-葡萄糖对人脐静脉内皮细胞增殖、迁移、管腔形成及细胞凋亡的影响，试图寻找治疗血管瘤的新药。方法用四甲基偶氮唑盐比色法、倒置显微镜下形态学观察、体外划痕实验的方法，观察2-脱氧-D-葡萄糖对人脐静脉内皮细增殖、迁移的作用；用成管法，检测2-DG对血管形成的影响；用流式细胞仪检测2-DG对血管内皮的凋亡影响、结果2-DG作用于人脐静脉内皮细胞后，对其增殖，迁移和成管能力有明显抑制作用，大大地增加细胞的凋亡率，且有显著剂量和时间依赖性．结论2-DG对HUVEC增殖、迁移和成管有显著的抑制作用，且能促使细胞凋亡。

Effect of 2-Deoxy.D-glucose on human umbilical vein endothelial cell

Objective To explore the effects of 2 Deoxy D glucose on the proliferation, migration, capil- lary formation and apoptosis of human umbilical vein endothelial cells （HUVECs）, searching a new medicine for hemangioma. Methods MTF ,morphorlogical test and scratch test were used to detect HUVECs proliferation rate and mirgrating ability after 2-DG treatment. Tube formation matrigel assay and flow cytometry were performed for the capillary formation and endothelial apoptosis after the drug intervention respectively. Results After 2-DG was applied to HUVECs, the proliferation, migration, tube formation of HUVECs were inhibited significantly. The apoptosis rate of these cells increased and was highly time and dosage dependent. Conclusion 2-DG inflicts negative effects on the proliferation and capillary formation of HUYECs, and induces apoptosis of HUVECs.