低密度脂蛋白和高密度脂蛋白对动脉内皮细胞一氧化氮生成的影响

为研究高密度脂蛋白和低密度脂蛋白对动脉内皮细胞一氧化氮合成的影响，以豚鼠动脉内皮细胞为实验对象，用低密度脂蛋白、高密度脂蛋白及两者以不同剂量混合加入培养基中，每组设置三种剂量，分别于２ｈ、６ｈ、１２ｈ和２４ｈ测定一氧化氮含量。结果发现，高密度脂蛋白促进内皮细胞一氧化氮的合成（Ｐ〉０．０５），与剂量大小无关；低密度脂蛋白显著抑制一氧化氮的合成（Ｐ〈０．００１），与剂量相关；混合组同样明显抑制其合成，

Effects of Low Density Lipoprotein and High Density Lipoprotein on Nitric Oxide Synthesized by Vascular Endothelial Cell

Aim To study nitric oxide (NO) variances after vascular endothial cell were impacted by low density lipoprotein (LDL) and high density lipoprotein (HDL). Methods Concentrations of NO in the cultured medium were detected in 2 h, 6 h, 12 h and 24 h after the addition of LDL, HDL and both of them respectively in the guinea pig vasular endothial cell. Results LDL could obviously decrease the NO synthesized by vasular endothial cell with dose depentent (P<0.01)and HDL could arise it (P<0.01), LDL mixed with three levels of HDL expressed the same effection as LDL alone (P>0.05). There was no difference in four time''s group (P>0.05). Conclusions NO synthesized by vasular endothial cell will be restrainted by LDL and arisen by HDL but couldn''t antagonist the former''s.