川芎嗪对血管平滑肌细胞增殖及表达c-myc基因的影响

为观察川芎嗪对血管平滑肌细胞增殖的影响，在建立凝血酶诱导的体外培养的兔主动脉血管平滑肌细胞增殖模型后，应用免疫细胞化学方法观察川芎嗪对血管平滑肌细胞表达ｃ－ｍｙｃ基因蛋白的影响；并用流式细胞术观察了血管平滑肌细胞增殖周期的变化。结果发现，川芎嗪能够显著抑制凝血酶诱导的血管平滑肌细胞ｃ－ｍｙｃ基因蛋白表达增加，使血管平滑肌处于ＧＩ期的细胞数显著增多，Ｓ期和Ｇ２＋Ｍ期的细胞数显著减少。结果提示，川芎嗪对凝血酶诱导的血管平滑肌细胞增殖有显著抑制作用，其机制与抑制ｃ－ｍｙｃ基因表达有关。

Effects of Tetramethylpyrazine on c-myc Gene Expression in Vascular Smooth Muscle Cells and Proliferation of Vascular Smooth Muscle Cells

Aim The effects of tetramethylpyrazine (TMP) on vascular smooth muscle cells (VSMC) proliferation were studied. Methods A cells proliferating model of rabbit aorta VSMC induced by thrombin were established; the effect of tetramethylpyrazine on c-myc gene expression in VSMC was observed by immunocytochemical method; the effect of tetramethylpyrazine on proliferation cycle of VSMC was observed by flow cytomytry technique. Results the highly expression of c-myc gene protein induced by thrombin can be inhibited by tetramethylpyrazine significantly, and the cells numbers of G1 phase were increased and the cells numbers of G1+M phase were decreased markedly. Conclusion The VSMC proliferation induced by thrombin can be inhibited by tetramethylpyrazine significantly, and the inhibited c-myc gene expression may contribute to the explanation of one of the mechanism of the inhibited VSMC proliferation.