重组人组织型纤溶酶原激活物对家兔实验性肺栓塞溶栓作用的初步研究

目的　观察重组人组织型纤溶酶原激活剂（ｒｔ－ ＰＡ）对兔肺栓塞的溶栓作用。方法　１２５Ｉ 标记人纤维蛋白原，体外制备１２５Ｉ 标记血栓，从颈静脉注入致肺栓塞。２－０ ｈ 内静滴２－１６，０－５４ 和０－２７ ｍｇ·ｋｇ－１ 的ｒｔ ＰＡ，与ＮＳ和２０ ０００ ＩＵ·ｋｇ－ １ 链激酶（ＳＫ） 比较。测定药物处置后心肺残留１２５Ｉ 血栓放射性评价药物的溶栓作用。结果　ｒｔ ＰＡ各组的溶栓率分别为５６ ％ ±２１％ ，４０ ％ ±１４％ ，２６ ％ ±１２％ ，ＮＳ和ＳＫ 组分别为６ ％ ±３ ％ 和４３ ％ ±１５％ 。给药组的溶栓率均显著高于ＮＳ对照；ｒｔ ＰＡ高剂量组明显高于低剂量组。给药前后出血时间和纤维蛋白原含量与ＮＳ对照无明显变化。结论　ｒｔ ＰＡ有依赖于剂量的明显溶栓作用

A preliminary study of thrombolysis by recombinant human tissue type plasminogen activator in rabbits with experimental pulmonary embolus

AIM To investigate the thrombolytic effect of recombinant human tissue type plasminogen activator (rt PA). METHODS An experimental pulmonary embolus was formed in rabbit by injecting 125 I labeled human fibrin blood clot through jugular vein. Thrombolytic effect was evaluated by (counting of 125 I labeled embolus remained in pulmonary arteries, or blood and urine containing the 125 I labeled degradation products after treatment. RESULTS Thrombolysis was observed after iv bolus and infusion of rt PA with 2 16, 0 54 and 0 27 mg·kg -1 over 2 0 h and compared with that of normal saline. The extent of thrombolysis was 56%, 40% or 26% after intravenous injection (0 72, 0 18 or 0 09 mg·kg -1 ) and infusion (0 72, 0 18 or 0 09 mg·kg -1 ·h -1 for 2 hours) of rt PA, respectively, which were significantly higher than that after normal saline (6%). The ratio of lysis and radioactivity of 125 I labeled degradation products in urine of rt PA with 2 16 mg·kg -1 were significantly higher than that with 0 27 mg·kg -1 . CONCLUSION rt PA resulted in dose-dependent thrombolysis.