成人隐匿性自身免疫性糖尿病患者血清中IL-10和TGF-β的变化及意义

目的探讨成人隐匿性自身免疫性糖尿病（LADA）患者外周血细胞因子白细胞介素-10（IL-10）、转化生长因子-β（TGF-β）的变化与意义。方法以双抗夹心ELISA法检测30例LADA、30例2型糖尿病（T2DM）、30例正常对照外周血血清IL-10、TGF-β水平。结果LADA组、2型糖尿病组的IL-10水平均显著高于正常对照组（P〈0．05），而LADA组低于2型糖尿病组（P〈0．05）。LADA组的TGF-β水平低于正常对照组（P〈0．05），但与2型糖尿病组相比没有统计学意义（P〉0．05）。结论LADA患者和2型糖尿病患者体内均存在着细胞免疫的紊乱。与2型糖尿病患者相比，LADA患者体内血清IL-10浓度降低，不能有效地维持自身的免疫耐受，可能是导致其胰岛β细胞功能加速衰退的重要因素。

Changes of IL-10 and TGF-βin adult patients with latent autoimmune diabetes

Objective To investigate the changes and significance of IL-10 and TGF-β in peripheral blood of latent autoimmune diabetes in adults （LADA） patients. Methods 30 LADA patients, 30 type 2 diabetic （T2DM） patients and 30 volunteers were involved in the study. Levels of IL-10 and TGF-β in peripheral blood were assessed by ELISA. Results The levels of IL-10 in LADA group and T2DM groups were significantly higher than that in normal control （ P 〈0. 05）, and the level of IL-10 in LADA group was lower than that in T2DM group （ P 〈 0. 05）. The level of TGF-β of LADA group was lower than that in normal control （ P 〈 0. 05 ）, but no significantly different compared with T2DM group. Conclusion There is imbalance of cell immune in LADA and diabetic patients. Concentration of IL-10 in LADA patients was lower than that in T2DM group, which can not effectively maintain autoimmune tolerance and lead to progressive demolition of β-cells.