静脉应用抑肽酶对大鼠肺移植模型缺血再灌注损伤的作用

目的 探讨静脉应用抑肽酶对肺移植后肺缺血再灌注损伤的作用和机制.方法 利用移植肺冷缺血14 h建立的大鼠肺移植缺血再灌注损伤模型,考察抑肽酶对缺血再灌注损伤的影响,并检测细胞因子等指标探讨机制.结果 抑肽酶组较对照组移植肺氧合好、湿干比小,同时支气管肺泡灌洗液中白细胞介素(IL)-2[(113±32)μg/L和(162±43)μg/L,P＜0.05]、血清中IL-8[(7.26±1.01)ng/L和(9.43±0.97)ng/L,P＜0.05]和肿瘤坏死因子(TNF)-α[(152.3±36.4)ng/L和(211.6±52.7)ng/L,P＜0.05]、肺组织中髓过氧化物酶活性[(2.36±0.62)U/g和(3.98±0.36)U/g,P＜0.05]都显著降低.结论 静脉应用抑肽酶能够减轻缺血再灌注损伤,机制可能包括:减少IL-2的释放、抑制TNF-α活化和IL-8产生,抑制中性粒细胞的聚集、激活和脱颗粒.

Intravenous aprotinin attenuates lung ischemia-reperfusion injury in a rat lung transplantation model

Objective To evaluate the effect of intravenous aprotinin application on ischemiareperfusion iniury after lung trailsplantation.Methods An isehemia-reperfusion model after lung transplantation in rats was established and received intravenous aprotinin.Cytokine levels and myeloperoxidase activity were measured in order to explore the mechanism.Rtsults Intravenous aprotinin attenuated ischemia-reperfusion injury estimated by blood gas and wet/dry ratio.The levels of IL-2 in bronchoalveolar lavage[(113±32)μg/L vs(162±43)μg/L,P＜0.05],serum levels of IL-8[(7.26±1.01)ng/Lvs(9.43±0.97)ng/L,P＜0.05]and TNF-α[(152.3±36.4)ng/L vs(211.6±52.7)ns/L,P＜0.05],and lung tissue myelopcroxidase activity[(2.36±0.62)U/g vs(3.98±0.36)U/g,P＜0.05]were significantly reduced in the aprotinin group.Conclusion Apmtinin attenuates lung ischemia-reperfusion iniury after lung transplantation by lessen the release of IL-2,inhibiting the activation of TNF-α and production of IL-8.and suppressing the accumulation,activation and deganulation of neutrophils.