京尼平交联明胶特性随时间变化的研究

研究了京尼平交联明胶材料的交联度、细胞毒性、溶胀度、降解率等特性随交联时间的变化.用1%京尼平交联明胶,按交联时间分为7组:10 min组、30 min组、1 h组、2 h组、12 h组、24 h组、72 h组.结果显示京尼平可有效交联明胶,随着交联时间延长,交联度增加,溶胀度和降解率降低.交联10 min的材料,交联度低(26.7%),溶胀度高(265%),不到一周就完全降解,说明材料很不稳定,易降解;交联30min的材料,交联度(45.7%)、溶胀度(207%)、降解率与10 min材料比都有明显变化,4周未完全降解,8周完全降解,说明京尼平可以在30 min内显著改变明胶性能;30 min后的材料,随交联时间延长,交联度逐渐增加,溶胀度和降解率逐渐降低.交联72 h的材料,交联度73.1%,溶胀度153%,12周仅降解15.6%.MTT法测各组材料细胞增殖率在87.9%～105.4%之间,说明京尼平交联明胶材料细胞毒性均很低.

Study on the Temporal Change of Properties of Genipin Crosslinked Gelatin

Investigated the changes of crosslinking index,swelling ratio,degradation rate and cytotoxicity of genipin crosslinked gelatin accompany with crosslinking time.1% genipin crosslinked gelatin were divided into 7 groups by crosslinking time: 10min group,30min group,1h group,2h group,12h group,24h group, 72h group.The results proved that genipin could crosslink gelatin effectively. Accompany with increasing of crosslinking time,crosslinking index increased,and swelling ratio,degradation rate decreased.In 10min group,crosslinking index was low(26.7%),swelling ratio was high,(265%),completely degraded within 1 week.This indicated that biomaterials of 10min group was instable and degraded easily.Compared with 10min group,biomaterials of 30min group changed significantly with crosslinking index(45.7%),swelling ratio(206%) and degration rate(completely degraded between 4 weeks and 8 weeks).This indicated that genipin could change the properties of gelatin within 30min.Biomaterials after 30min,crosslinking index increased,and swelling ratio,degradation rate decreased gradually accompanied with increasing of crosslinking time.Biomaterials of 72h,crosslinking index was 73.1%,swelling ratio was 152%,and degradated 18.9% after 12 weeks.RGR(relative cell growth rate) of every group measured by MTT assay changed between 87.9% and 105.4%,indicated that the cytotoxicity of genipin crosslinked gelatin was very low.