金樱子提取液对H2O2诱导HaCaT细胞氧化损伤保护作用研究

目的探讨金樱子提取液对H2O2诱导后人角质形成细胞(HaCaT cell)氧化损伤的保护作用。方法体外培养人角质形成细胞,用不同浓度的H2O2诱导细胞构建氧化应激损伤模型。CCK-8法检测不同浓度金樱子提取液对细胞增殖作用的影响;Hochest染色法检测不同浓度金樱子提取液对细胞凋亡作用的影响。结果100μmol·L-1 H2O2为构建氧化应激损伤模型的最适浓度。CCK-8法检测结果表明金樱子提取液能显著提高细胞的增殖,且随浓度的升高而增大。Hochest染色结果表明金樱子提取液能有效抑制由H2O2氧化损伤引起的细胞凋亡。结论金樱子提取液对H2O2诱导后的人角质形成细胞具有一定的氧化损伤保护作用。

Study on the protective effect of extracts from Rosa laevigata Mickx.on H2O2-induced oxidative damage of HaCaT cells

Objective To explore the protective effect of extracts of Rosa laevigata Mickx.(RLM)on H2O2-induced oxidative damage of HaCaT cells.Methods Different concentrations of H2O2were used to induce HaCaT cell.CCK-8 method was used to detect the protective effect of different concentrations of RLM extracts on HaCaT cells under H2O2stimulation,and apoptosis was detected by Hochest staining.Results CCK-8 test results showed that 100μmol·L-1 H2O2was the most suitable concentration for constructing damage HaCaT model.Compared with control group,the cell viability of different concentrations of RLM extracts increased significantly in a dose dependent manner.The results of Hochest staining showed that RLM extract effectively inhibited apoptosis caused by H2O2oxidative injury,and the inhibition effect increased with the increase of concentration.Conclusion RLM extracts showed a significant antioxidant activity in HaCaT cells induced by H2O2.