HPLC测定川西合耳菊中黄酮类和酚酸类药效成分的含量

目的建立藏药川西合耳菊Synotis solidaginea中黄酮类和酚酸类药效成分含量测定的方法,并评价不同产地、不同部位样品的质量。方法建立HPLC测定川西合耳菊中芦丁、金丝桃苷、异槲皮苷、烟花苷、紫云英苷、绿原酸、异绿原酸A、异绿原酸C 8种有效成分含量的方法。从西藏、四川和青海收集了19份川西合耳菊药材样品;将全株植物分成茎、叶、花3个部位,测定这些样品中8种成分的含量。运用主成分分析法比较不同产地药材中的药效成分,以及植物不同部位之间和同一部位不同产地样品之间差异。结果这8种药效成分在40 min内分离良好,加样回收率为96.3%～104.9%。19份川西合耳菊样品中3种酚酸类成分的总量为5种黄酮类成分总量的2.77倍。酚酸类以绿原酸的含量最高(9.74 mg/g);黄酮类以芦丁的含量最高(3.65 mg/g)。根据药效成分含量和主成分分析,四川道孚的样品质量更好。植物3个部位之间比较,总量以叶中含量最高,占59.15%～65.61%;其次为花,占30.52%～34.31%;茎中的含量最低,仅占3.9%～5.7%。金丝桃苷在花中的含量高,而其他7种成分在叶中的含量最高。结论建立的方法能准确测定川西合耳菊中5种黄酮类和3种酚酸类成分的含量,评价其质量。川西合耳菊叶和花的药效成分含量高,应于开花期、叶茂盛时采集,并防止叶和花脱落,以保证质量。

Determination of bioactive compounds belonging to flavonoids and phenolic acids in Synotis solidaginea by HPLC

Objective To develop a method for quantifying the bioactive compounds belonging to the types of flavonoids and phenolic acids in the herb of Synotis solidaginea, and to assess the quality of this herbal samples among different cultivating areas and plant parts. Methods An HPLC method was developed to quantify the amounts of eight bioactive compounds, i.e. rutin, hyperoside, isoquercitrin, nicotiflorin, astragalin, chlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C in this herb. A total of 19 herbal samples were collected from Tibet, Sichuan, and Qinghai in China. The whole plant was divided into three parts including stem, leaf, and flower. The amounts of the eight bioactive compounds in these samples were quantified. Principal component analysis (PCA) was used to compare the bioactive compounds in this herbal samples among different cultivating areas, various plant parts and same plant parts collected from different cultivating areas. Results The eight peaks of these bioactive components were separated well within 40 min. The recoveries of the eight compounds were 96.3%—104.9%. The total amount of three phenolic acids in 19 herbal samples was 2.77 folds of five flavonoids. The content of chlorogenic acid was the highest in phenolic acids (9.74 mg/g). The highest content among flavonoid compounds was rutin (3.65 mg/g). Both the content of bioactive components and the result of PCA indicated that the quality of herbal samples collected from Daofu of Sichuan was best among the three cultivation areas. Comparing the total amounts of eight bioactive compounds among the three parts of this plant, leaf was top one accounting for 59.15%—65.61%, flower was second accounting for 30.52%—34.31%, stem was third only accounting for 3.9%—5.7%. More amount of hyperoside was found in flowers, whilst the other seven components were found in high levels in leaves. Conclusion The developed method is accurate to quantifying the levels of five flavonoids and three phenolic acids in this herb and can be used to assess its quality. The leaves and flowers of this herb contain high amount of bioactive components. This herb should be harvested during flowering and flourishing leaves. Moreover, the leaves and flowers should be prevented from lost for quality assurance.