钳蝎蝎毒组分Ⅱ对人直肠腺癌C1184细胞生长的影响

蝎毒组分Ⅱ（ＳｃｏｒｐｉｏｎＶｅｎｏｍｃｒｕｄｅⅡ，ＳＶＣⅡ）为河南马氏钳蝎粗毒（ＳＶＣ）经分离纯化后获得的有效成分之一。实验结果表明，以丝裂霉素作为阳性对照，ＳＶＣⅡ（４～２０μｇ／ｍｌ）对人直肠腺癌Ｃ１１８４细胞株有明显杀伤作用。与空白对照相比，ＳＶＣⅡ使细胞生长抑制率、细胞毒性和克隆形成抑制率增大（Ｐ＜０．０５或（Ｐ＜０．０１），并存在剂量效应关系，其半数抑制浓度分别为１１．２０μｇ／ｍｌ、１１．９３μｇ／ｍｌ和１７．３８μｇ／ｍｌ。从生长曲线可见，ＳＶＣⅡ对细胞生长呈剂量和时间依赖性抑制。ＳＶＣⅡ还能使细胞分裂指数下降。提示ＳＶＣⅡ具有抗癌细胞增殖作用。

The effect of the component Ⅱ in scorpion venom crude extracted from buthus martensii karsch(svc Ⅱ)on the human rectum adenocarcinoma C1184 cell line

Scorpion Venom Crude Ⅱ(SVC Ⅱ)was one of the effective components which was isolated from the scorpion venom crude extracted from Buthus Martensii Karsch in Henan Province.The experimentalresults showed that SVC Ⅱ with the concentration ranging from 4μg/ml to 20μg/ml had obvious killing acticitv on the human rectum adenocarcinoma C1184 cell line in Vitro in comparison with the positive control which was Mitomycin-c.Compared with the blank control,SVC Ⅱcould induceto increase the inhibitary rate of cell growth,the cvtotoxicitv and inhibited rate of the colonv formation(P0.05 or P0.01).There existed the dose-response correlations between the inhibitioneffect and the dose of SVC Ⅱ.The half inhibition concentrations of cell growth(Ⅰ C50)in above experiments were 11.20μg/ml、11.93μg/ml and 17. 38μg/ml,respectively.It was found that SVCⅡ signficantly inhibited C1184 cell growth in the dose-dependent and time-dependent manner in cell growth curve.The mitotic index of C1184 cells could be reduced by SVC Ⅱ.So it was supported that.SVC Ⅱpossess antineoplastic effect on tumor cell proliferation.