人乳头瘤病毒、人巨细胞病毒和单纯疱疹病毒感染与宫颈癌的关系

目的　本研究旨在探讨宫颈癌前病变和宫颈癌的发生发展与人乳头状瘤病毒及HSV、CMV的关系。方法　对81例不同宫颈病变组织进行HPV16 /18和HPV6 /11原位杂交,同时对98例不同宫颈病变组织用DNA扩增法检测HPV、HSV和CMV。结果　病毒DNA原位杂交信号的分布与HE染色中挖空细胞的分布一致。HPV16 /18与不同宫颈病变组织原位杂交阳性率平均为5 1 .1% ,HPV6 /11的则为6 4 .7%。HPV16 /18、HPV6 /11、HSV、CMV在不同宫颈病变组织中的阳性率分别为2 1%、4 %、2 3%、0 %。结论　HPV感染具有特定的组织学部位,HSV可协同HPV16 /18恶性转化宫颈上皮细胞。

Relationship between infection of HPV, HSV, CMV and cervical cancer

Objective The carcinogenesis of the human cervical precancerous lesion, cervical carcinoma is known closely associated with human papillomavirus(HPV). The purpose of this article is to identify whether HSV and CMVplay a co-factor role in the carcinogenesis. Methods Eighty-one cases of various cervical lesions were analyzed by HPV6/11,HPV16/18 in situ hybridization. Meanwhile, HPV, HSV and CMV were determined in 98 cases of various cervical lesions. Results The results show that the distribution of positive hybridization signal was consistent with the distribution of koilocytes in HE stain. Of these cervical specimens investigated, the positive rates of HPV16/18 and HPV6/11 using ISH were 51.1% and 64.7%, respectively, the infection rates of HPV16/18, HPV6/11, HSV and CMV using PCR were 21%, 4%, 23% and 0%, respectively. Conclusion The co-operation effect of HPV and HSV occurred in the oncogenesis of human cervical carcinoma.