广州管圆线虫感染大理州福寿螺模型的构建及探讨

目的 构建广州管圆线虫人工感染大理州福寿螺实验动物模型,观察并探讨感染后福寿螺的生长变化和感染效果,为本地区深入研究广州管圆线虫病提供充足的试验材料.方法 用福寿螺体内的广州管圆线虫(第三期幼虫)感染昆明小鼠,6周后用含有广州管圆线虫一期幼虫的新鲜鼠粪感染大理本地繁殖的福寿螺,将福寿螺随机分为A、B、C、D组,每组40...

Construction and discussion of an infection model of Dali Ampullaria gigas by Angiostrongylus cantonensis

Objective To set up an artificial infection model of Dali Ampullaria gigas by Angiostrongylus cantonensis, observe the growth after infection and the effectiveness of the infection, thus to provide adequate materials for further research on angiostrongyliasis cantonensis in Dali. Methods Kunming mice were infected with Angiostrongylus cantonensis selected from positive Ampullaria gigas. After 6 weeks of infection,the first stage larvae of Angiostrongylus cantonensis in fresh mice feces were collected to infect Ampullaria gigas of Dali. Ampullaria gigas were randomly divided into 4 groups, namely A, B, C, D (40 per group). Ampullaria gigas in group A, B, C, D were infected with 0, 800,700, 600 first stage larvae of Angiostrongylus cantonensis respectively,keeping infecting for 7 days. On 36 ～40 d after infection the third stage larvae were collected by pepsin digestion to determine the death rate, infection rate, infectiosity of Ampullaria gigas, and infectivity of the third stage larvae to infect Kunming mice. Results The mortality of Ampullaria gigas in group A, B, C, D were 0, 65%, 60%, 25% respectively. The infection rate in group A was 0 while that in group B, C, D was 100%. Meanwhile, the infectiosity in group D was higher than that in group B and C(P＜0.05). The third stage larvae separated from each group could infect Kunming mice successfully with the infection rates of 100%. Conclusion Angiostrongylus cantonensis could successfully infect Ampullaria gigas from Dali. The third stage larvae from Dali Ampullaria gigas could infect Kunming mice.