子痫前期患者血清中sEng水平的研究

目的通过对子痫前期患者血清中的可溶性Endoglin（soluble Endoglin,sEng）的表达水平的测定,探讨其与子痫前期发病的关系及意义。方法选取2008年10月~2009年10月在泸州医学院附属医院产科住院分娩的子痫前期患者40例与同期健康孕妇40例作为对照组。采用酶联免疫吸附试验（ELISA）夹心法检测子痫前期患者与正常妊娠妇女血清中的sEng的表达水平。采用SPSS 14.0统计软件包进行统计学分析,数据以均数±标准差（x-±s）表示;两样本均数比较用随机独立样本均数t检验。取α=0.01作为检验水平,P〈0.01差异有统计学意义。结果子痫前期组血清中sEng为7.38±1.78 ng/ml,正常妊娠组为2.17±0.59 ng/ml,两组比较差异有显著性（P〈0.01）。重度痫前期组血清中sEng为7.94±1.27 ng/ml,轻度痫前期组血清中sEng为5.72±1.09 ng/ml,重度子痫前期与轻度子痫前期比较,差异有显著性（P〈0.01）。血清中sEng的水平随疾病病情的加重而升高。结论 sEng在子痫前期患者的外周血中高表达,sEng水平升高的程度能与疾病的严重程度相关。

Studies of serum sEng levels and the expression of Endoglin in placenta in patients with preeclampsia

Objective To investigate the roles and significance in patients with preeclampsia with observing the level of soluble Endoglin（sEng） in patients with preeclampsia.Methods 40 patients were enrolled in the present study.Among the 40 patients,10 cases were mild preeclampsia and 30 cases were sever preeclampsia.40 healthy pregnant women were selected as the control group over the same period.The serum sEng was measured with enzyme-linked immunosorbent assay（ELISA）.Results The serum sEng was 7.38±1.78 ng/ml in preeclampsia group and 2.17±0.59ng/ml in normal group.Serum sEng in patients with preeclampsia was significantly higher than those in controls（P0.01）.The level of sEng in maternal serum was 7.94±1.27ng/ml in sever preeclampsia group and 5.72±1.09 ng/ml in mild preeclampsia group.The sEng levels were higher in severe preeclampsia compared to mild.Serum sEng levels increased in accordance with the severity of preeclampsia.Conclusion Serum sEng levels is significantly increased in patients with preeclampsia and increased with the severity degree of diseases.