中华鲟软骨制剂抑制血管内皮细胞迁移的实验研究

目的：探讨中华鲟软骨活性成分体外抑制血管内皮细胞迁移的作用.方法：应用丙酮沉淀和超滤法抽提和分离中华鲟软骨活性成分,通过不同分子量的软骨溶液作用于内皮细胞,计算迁移抑制率,分析中华鲟软骨活性成分对内皮细胞迁移的抑制作用.结果：中华鲟软骨溶液Ⅰ和溶液Ⅲ对血管内皮细胞迁移的抑制率分别是46.14%和76.35%,与Ⅳ组比较差异显著（t=3.83和2.91,P＜0.05＝;Ⅱ组抑制率是5.64%与Ⅳ组比较差异不显著（t=1.87,P＞0.05）;中华鲟软骨溶液Ⅲ与v组（57.32%）比较差异显著（t=2.45,P＜0.05＝.结论：中华鲟软骨活性成分可以抑制血管内皮细胞迁移.

Experimental study for the inhibition effect of Chinese sturgeon cartilage tissue on the migration of vascular endothelial cell

Objective：To investigate the inhibition effect of Chinese sturgeon cartilage tissue on the migration of vascular endothelial cell. Methods：Chinese sturgeon cartilage active tissue was extracted and separated by ways of acetone precipitation and ultrafiltration. Vascular endothelial cell was acted by different solution. We investigate the inhibition effect of Chinese sturgeon cartilage active tissue on the migration of the vascular endothelial cell by inhibition ratio of migration. Results：Inhibition ratio of Chinese sturgeon cartilage solution i and solution iii that acted vascular endothelial cell were 46.14% and 76.35%. They were significantly different than solution iv （t=3.83 and 2.91 ,P 〈0.05） ;Inhibition ratio of Chinese sturgeon cartilage solution ii was 5.64% that was not significant than solution iv （t= 1.87,P〉0.05） ;And inhibition ratio of solution iii was significantly different than that of solution v （t = 2.45 ,P 〈0.05）. Conclusion：Chinese sturgeon cartilage active tissue can inhibit migration of vascular endothelial cell.