| MALDI-TOF-MS分析新疆食管鳞状细胞癌的蛋白组学 |
| |
| 引用本文： | 吕秀平,蒲红伟,龚晓瑾,苗娜,陈晓. MALDI-TOF-MS分析新疆食管鳞状细胞癌的蛋白组学[J]. 世界华人消化杂志, 2011, 0(36) |
| |
| 作者姓名： | 吕秀平 蒲红伟 龚晓瑾 苗娜 陈晓 |
| |
| 作者单位： | 新疆医科大学病理学教研室;新疆医科大学第二附属医院病理科;新疆医科大学第一附属医院病理科; |
| |
| 基金项目： | 新疆地方病分子生物学实验室开放基金资助项目,No.XJDX0208-2006-09~~ |
| |
| 摘 要： | 目的:分析新疆食管鳞状细胞癌和食管正常上皮细胞的差异表达蛋白.方法:运用激光捕获显微切割技术(LCM)分别获取食管鳞状癌细胞和食管正常上皮细胞,应用二维凝胶电泳技术(2-DE)分离纯化细胞,Imagemaster 2D软件比较分析两者电泳图谱的差异,基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)鉴定分析两者表达的差异蛋白.结果:建立了食管鳞状癌细胞和食管正常上皮细胞的2-DE图谱,获得43个差异蛋白点,通过质谱鉴定出17种蛋白,其中15种蛋白如Trangelin2、HSP27、S100A11、GSTP等在食管鳞状癌细胞中表达明显增高,2种蛋白如SCCA1,在食管鳞状癌细胞中表达明显降低.结论:提示17种差异蛋白可能与食管鳞状细胞癌的发生和发展有关,为筛选食管鳞状细胞癌的特异性分子标志物奠定基础.
|
| 关 键 词： | 食管鳞状细胞癌 蛋白组学 差异表达蛋白 二维凝胶电泳 基质辅助激光解吸电离飞行时间质谱 |
Proteomic analysis of differentially expressed proteins in esophageal squamous cell carcinoma by MALDI-TOF-MS |
| |
| Xiu-Ping Lv,Hong-Wei Pu,Xiao Chen. Proteomic analysis of differentially expressed proteins in esophageal squamous cell carcinoma by MALDI-TOF-MS[J]. World Chinese Journal of Digestology, 2011, 0(36) |
| |
| Authors: | Xiu-Ping Lv Hong-Wei Pu Xiao Chen |
| |
| Affiliation: | Xiu-Ping Lv,Hong-Wei Pu,Xiao Chen,Department of Pathology,Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China Xiao-Jin Gong,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830063,China Na Miao,the First Affiliated Hospital of Xinjiang Medical University,China |
| |
| Abstract: | AIM:To analyze differentially expressed proteins between human normal esophageal epithelial cells (NEEC) and esophageal squamous cell carcinoma (ESCC) cells.METHODS:NEEC and ESCC cells were cut by laser capture microdissection (LCM),and total proteins of the cells were separated by twodimensional gel electrophoresis (2-DE).2-DE images were analyzed using Imagemaster 2D software.Differential proteins between NEEC and ESCC cells were identified by matrix-assisted laser desorption ionization time-of-flight mas... |
| |
| Keywords: | Esophageal squamous cell carcinoma Proteomics Differential proteins Two-dimensional gel electrophoresis Matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
| 本文献已被 CNKI 等数据库收录！ |
|
