| KDR单克隆抗体对荷人胃癌裸小鼠肿瘤生长的抑制作用 |
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| 引用本文： | 孔桂美,张小荣,吴克艳,赵飞燕,朱海杭,张旭东,廖月霞,卜平. KDR单克隆抗体对荷人胃癌裸小鼠肿瘤生长的抑制作用[J]. 世界华人消化杂志, 2011, 0(28) |
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| 作者姓名： | 孔桂美 张小荣 吴克艳 赵飞燕 朱海杭 张旭东 廖月霞 卜平 |
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| 作者单位： | 扬州大学临床医学院;扬州大学; |
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| 基金项目： | 国家自然科学基金资助项目,No.30801497~~ |
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| 摘 要： | 目的:研究血管内皮细胞生长因子(vascularendothelialgrowthfactor,VEGF)受体Ⅱ单克隆抗体(KDR-mAb)抑制荷人胃癌裸小鼠肿瘤生长的作用.方法:将人胃癌细胞(SGC-7901)通过背部皮下接种裸小鼠制备荷人胃癌小鼠肿瘤模型.待肿瘤生长至直径100-300mm3时将荷瘤小鼠随机分为3组,分别腹腔注射KDR-mAb、5-FU和生理盐水进行治疗.10d后剥离瘤组织,比较3组肿瘤组织的体积和质量、计算抑瘤率;通过Real-timePCR法和免疫组织化学法分别定量检测VEGF在各治疗组肿瘤组织中的表达.结果:KDR-mAb在体内能显著抑制实体瘤的生长,抑瘤率为36.3%,肿瘤大小和肿瘤质量与对照组比较差异显著(1889.94mm3±396.64mm3vs9398.34mm3±7413.96mm3,1.07g±0.58gvs1.68g±0.18g,均P<0.05);KDR-mAb能显著抑制肿瘤组织中VEGFmRNA和VEGF蛋白的表达(P<0.05).结论:作为VEGF受体抑制剂,KDR-mAb在荷人胃癌细胞小鼠肿瘤模型中具有明显的体内抗肿瘤活性,对于人胃癌的临床治疗具有一定的应用前景...
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| 关 键 词： | 血管内皮细胞生长因子受体Ⅱ 单克隆抗体 胃癌 抗肿瘤活性 |
Inhibitory effect of KDRspecific monoclonal antibody on tumor growth in nude mice bearing human gastric cancer |
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| Gui-Mei Kong,Ping Bo,Clinical Medical School of Yangzhou University,Yangzhou ,Jiangsu Province,ChinaXiao-Rong Zhang,Ke-Yan Wu,Fei-Yan Zhao,Yue-Xia Liao,Yangzhou University,ChinaHai-Hang Zhu,Xu-Dong Zhang,China. Inhibitory effect of KDRspecific monoclonal antibody on tumor growth in nude mice bearing human gastric cancer[J]. World Chinese Journal of Digestology, 2011, 0(28) |
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| Authors: | Gui-Mei Kong Ping Bo Clinical Medical School of Yangzhou University Yangzhou Jiangsu Province ChinaXiao-Rong Zhang Ke-Yan Wu Fei-Yan Zhao Yue-Xia Liao Yangzhou University ChinaHai-Hang Zhu Xu-Dong Zhang China |
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| Affiliation: | Gui-Mei Kong,Ping Bo,Clinical Medical School of Yangzhou University,Yangzhou 225001,Jiangsu Province,ChinaXiao-Rong Zhang,Ke-Yan Wu,Fei-Yan Zhao,Yue-Xia Liao,Yangzhou University,ChinaHai-Hang Zhu,Xu-Dong Zhang,China |
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| Abstract: | AIM:To evaluate the anti-cancer activity of monoclonal antibody(mA) against vascular endothelial growth factor(VEGF) receptor II(KDR) in nude mice bearing human gastric cancer.METHODS:BALB/c nude mice were subcutaneously inoculated with human gastric cancer cells(SGC-7901) to develop a xenograft model of human gastric cancer.When tumor volumereached 100-300 mm3,all mice were randomly divided into three groups and intraperitoneally injected with KDR-mAb,5-FU and physiological saline,respectively.Ten days aft... |
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| Keywords: | Vascular endothelial growth factor receptor II Monoclonal antibody Gastric cancer Anti-cancer activity |
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