| 野生型p53对肝癌细胞POLD1基因表达及细胞恶性表型的影响 |
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| 引用本文： | 韦长元,刘起理,廖柳凤,徐恒,谭晓虹.野生型p53对肝癌细胞POLD1基因表达及细胞恶性表型的影响[J].世界华人消化杂志,2011(23):2443-2449. |
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| 作者姓名： | 韦长元 刘起理 廖柳凤 徐恒 谭晓虹 |
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| 作者单位： | 广西医科大学附属肿瘤医院;广西医科大学医学实验中心; |
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| 基金项目： | 国家自然科学基金资助项目,No.303296~~ |
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| 摘 要： | 目的:探讨野生型p53对人肝癌细胞SMMC-7721细胞增殖和细胞恶性表型影响的一种可能机制,方法:设计并构建p53特异性小干扰shRNA绿色荧光真核表达质粒(p53-siRNA)和表达EGFP-p53融合蛋白的p53绿色荧光真核增强表达质粒(pEGFP-p53),通过脂质体Lipofection-2000介导转染,将...
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| 关 键 词： | SMMC-7721 POLDl p53 RNA干扰 |
Effect of overexpression of wild-type p53 on POLD1 expression and malignant cell behavior in human hepatocellular carcinoma cell line SMMC-7721 |
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| Chang-Yuan Wei,Qi-Li Liu,Liu-Feng Liao,Xiao-Hong Tan,Cancer Hospital of Guangxi Medical University,Nanning ,Guangxi Zhuang Autonomous Region,China Heng Xu,Experimental Center of Medical Sciences,Guangxi Medical University,China.Effect of overexpression of wild-type p53 on POLD1 expression and malignant cell behavior in human hepatocellular carcinoma cell line SMMC-7721[J].World Chinese Journal of Digestology,2011(23):2443-2449. |
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| Authors: | Chang-Yuan Wei Qi-Li Liu Liu-Feng Liao Xiao-Hong Tan Cancer Hospital of Guangxi Medical University Nanning Guangxi Zhuang Autonomous Region China Heng Xu Experimental Center of Medical Sciences Guangxi Medical University China |
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| Institution: | Chang-Yuan Wei,Qi-Li Liu,Liu-Feng Liao,Xiao-Hong Tan,Cancer Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China Heng Xu,Experimental Center of Medical Sciences,Guangxi Medical University,China |
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| Abstract: | AIM:To investigate the impact of overexpression of wild-type p53 on cell proliferation and malignant phenotype in human hepatocellular carcinoma cell line SMMC-7721 and to explore possible mechanism involved.METHODS:Enhanced green fluorescence protein gene-containing eukaryotic expressionplasmids expressing p53-specific small interfering RNA(shRNA)(p53-siRNA) or wild-type p53(pEGFP-p53) were constructed and introduced into SMMC-7721 cells by Lipofection2000-mediated transfection.Meanwhile,the pEGFP-C1 empty... |
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| Keywords: | SMMC-7721 POLD1 p53 RNA interference |
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